| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATF7IP2 |
| Uniprot No | Q5U623 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1–682aa |
| 氨基酸序列 | MASPDRSKRKILKAKKTMPLSCRKQVEMLNKSRNVEALKTAIGSNVPSGNQSFSPSVITRTTEITKCSPSENGASSLDSNKNSISEKSKVFSQNCIKPVEEIVHSETKLEQVVCSYQKPSRTTESPSRVFTEEAKDSLNTSENDSEHQTNVTRSLFEHEGACSLKSSCCPPSVLSGVVQMPESTVTSTVGDKKTDQMVFHLETNSNSESHDKRQSDNILCSEDSGFVPVEKTPNLVNSVTSNNCADDILKTDECSRTSISNCESADSTWQSSLDTNNNSHYQKKRMFSENEENVKRMKTSEQINENICVSLERQTAFLEQVRHLIQQEIYSINYELFDKKLKELNQRIGKTECRNKHEGIADKLLAKIAKLQRRIKTVLLFQRNCLKPNMLSSNGASKVANSEAMILDKNLESVNSPIEKSSVNYEPSNPSEKGSKKINLSSDQNKSVSESNNDDVMLISVESPNLTTPITSNPTDTRKITSGNSSNSPNAEVMAVQKKLDSIIDLTKEGLSNCNTESPVSPLESHSKAASNSKETTPLAQNAVQVPESFEHLPPLPEPPAPLPELVDKTRDTLPPQKPELKVKRVFRPNGIALTWNITKINPKCAPVESYHLFLCHENSNNKLIWKKIGEIKALPLPMACTLSQFLASNRYYFTVQSKDIFGRYGPFCDIKSIPGFSENLT |
| 预测分子量 | 81.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATF7IP2重组蛋白的示例参考文献(部分信息可能为虚构，建议通过学术数据库验证)：
1. **文献名称**: *ATF7IP2 regulates MYST-mediated histone acetylation and gene transcription*
**作者**: Smith J, et al.
**摘要**: 研究通过重组ATF7IP2蛋白的体外实验，揭示了其与MYST家族组蛋白乙酰转移酶(如TIP60)的相互作用，证明ATF7IP2在染色质修饰和转录激活中的关键作用。
2. **文献名称**: *Recombinant ATF7IP2 promotes cell proliferation via ERK signaling in colorectal cancer*
**作者**: Zhang L, et al.
**摘要**: 利用重组ATF7IP2蛋白处理结肠癌细胞，发现其通过激活ERK通路促进细胞增殖，提示ATF7IP2可能作为癌症治疗的潜在靶点。
3. **文献名称**: *Structural characterization of ATF7IP2 and its role in chromatin remodeling*
**作者**: Jones R, et al.
**摘要**: 通过X射线晶体学解析重组ATF7IP2蛋白的结构，发现其C端结构域介导与核蛋白的结合，为理解其在染色质重塑复合体中的功能提供结构基础。
4. **文献名称**: *ATF7IP2 knockdown alters DNA damage response in human fibroblasts*
**作者**: Tanaka K, et al.
**摘要**: 研究使用重组ATF7IP2蛋白进行功能补偿实验，证明其参与DNA损伤修复通路，并与ATR激酶活性调控相关。
**注意**：以上文献为示例性质，实际研究中可能需要通过PubMed、Google Scholar等平台以“ATF7IP2 recombinant protein”或“ATF7IP2 MEAF6”等关键词检索最新成果。若文献不足，可扩展至该蛋白的家族成员(如ATF7IP或MEAF6)相关研究。
ATF7IP2 (Activating Transcription Factor 7 Interacting Protein 2) is a protein-coding gene implicated in transcriptional regulation and chromatin remodeling. The encoded protein interacts with various transcriptional modulators, including the SET domain bifurcated histone lysine methyltransferase 1 (SETDB1), a key enzyme responsible for histone H3 lysine 9 trimethylation (H3K9me3), a hallmark of transcriptionally repressive chromatin. This interaction suggests ATF7IP2 plays a role in epigenetic silencing, particularly in regulating retrotransposons and maintaining genomic stability. Dysregulation of ATF7IP2 has been associated with neurodevelopmental disorders and cancers, highlighting its potential as a therapeutic target or biomarker.
Recombinant ATF7IP2 protein is engineered for functional studies to elucidate its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant protein retains functional domains necessary for binding partners like SETDB1. Researchers utilize it to investigate its role in chromatin dynamics, gene silencing, and retroelement suppression. Additionally, it serves as a tool for screening inhibitors or activators of ATF7IP2-SETDB1 interactions, which may have implications for treating diseases linked to epigenetic dysregulation. Structural analyses of recombinant ATF7IP2 also aid in mapping interaction interfaces and understanding its regulatory network. Despite progress, its full functional spectrum and tissue-specific roles remain under exploration, necessitating further studies using recombinant tools.
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