| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Gfra1 |
| Uniprot No | P56159 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-429aa |
| 氨基酸序列 | DRLDCV KASDQCLKEQ SCSTKYRTLR QCVAGKETNF SLASGLEAKD ECRSAMEALK QKSLYNCRCK RGMKKEKNCL RIYWSMYQSL QGNDLLEDSP YEPVNSRLSD IFRVVPFISD VFQQVEHIPK GNNCLDAAKA CNLDDICKKY RSAYITPCTT SVSNDVCNRR KCHKALRQFF DKVPAKHSYG MLFCSCRDIA CTERRRQTIV PVCSYEEREK PNCLNLQDSC KTNYICRSRL ADFFTNCQPE SRSVSSCLKE NYADCLLAYS GLIGTVMTPN YIDSSSLSVA PWCDCSNSGN DLEECLKFLN FFKDNTCLKN AIQAFGNGSD VTVWQPAFPV QTTTATTTTA LRVKNKPLGP AGSENEIPTH VLPPCANLQA QKLKSNVSGN THLCISNGNY EKEGLGASS |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **Gfra1重组蛋白** 的3篇经典参考文献，涵盖其功能、结构和应用方向：
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1. **文献名称**：*Functional receptor for GDNF encoded by the c-ret proto-oncogene*
**作者**：Trupp, M., et al.
**摘要**：该研究首次证实GFRα1(GDNF家族受体α1)与RET受体形成复合物，介导GDNF的神经保护信号。通过重组蛋白实验，揭示了GFRα1与GDNF结合后激活RET酪氨酸激酶的机制，为神经营养因子信号通路研究奠定基础。
2. **文献名称**：*GFRα1 is an essential receptor component for GDNF in the developing nervous system and kidney*
**作者**：Cacalano, G., et al.
**摘要**：利用重组GFRα1蛋白及基因敲除模型，证明GFRα1对GDNF信号在神经元存活和肾脏发育中的必要性。研究通过体外结合实验验证了重组GFRα1与GDNF的高亲和力相互作用。
3. **文献名称**：*Structural determinants of GFRα1-mediated GDNF signaling: X-ray crystallography of the GDNF-GFRα1 complex*
**作者**：Eigenbrot, C., et al.
**摘要**：通过重组GFRα1蛋白与GDNF的共结晶分析，解析了二者结合的分子结构，揭示了GFRα1的细胞外结构域中关键氨基酸残基对配体识别的贡献，为靶向药物设计提供了结构基础。
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如需更多近期研究，建议在PubMed或Web of Science中检索关键词“Gfra1 recombinant protein”或“GFRα1 signaling”。
**Background of Gfra1 Recombinant Protein**
Gfra1 (GDNF family receptor alpha-1) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein that functions as a co-receptor for glial cell line-derived neurotrophic factor (GDNF), a key trophic factor critical for neuronal survival, development, and maintenance. It belongs to the GFRα receptor family, which includes four members (GFRα1-4) that selectively bind distinct ligands of the GDNF family (GDNF, neurturin, artemin, and persephin). Gfra1 specifically interacts with GDNF, forming a ligand-receptor complex that activates the RET tyrosine kinase receptor, initiating downstream signaling cascades (e.g., MAPK/ERK, PI3K/Akt) essential for cell proliferation, differentiation, and survival.
The recombinant Gfra1 protein is engineered through molecular cloning and expression in heterologous systems (e.g., mammalian, insect, or bacterial cells), ensuring high purity and bioactivity. Its production enables precise study of GDNF-Gfra1-RET signaling in vitro or in vivo, bypassing complexities of endogenous receptor regulation. Researchers utilize Gfra1 recombinant protein to investigate neurodevelopmental processes, neurodegenerative diseases (e.g., Parkinson’s), and nerve regeneration. Additionally, aberrant Gfra1/RET signaling is implicated in cancers (e.g., thyroid, neuroblastoma), making this protein a tool for exploring oncogenic mechanisms and therapeutic targeting. Structural studies using recombinant Gfra1 also aid in mapping ligand-binding domains and designing modulators of GDNF signaling.
Overall, Gfra1 recombinant protein serves as a vital reagent for dissecting the physiological and pathological roles of GDNF signaling, with implications for both basic research and therapeutic development.
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