| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GYPB |
| Uniprot No | P06028 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-91aa |
| 氨基酸序列 | LSTTEVAMHTSTSSSVTKSYISSQTNGETGQLVHRFTVPAPVVIILIILCVMAGIIGTILLISYSIRRLIKA |
| 预测分子量 | 10.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GYPB重组蛋白的3篇参考文献(信息基于公开研究内容概括，非真实文献)：
---
1. **文献名称**: *"Expression and Characterization of Recombinant Glycophorin B in Mammalian Cells"*
**作者**: Smith A, et al.
**摘要**: 研究报道了利用哺乳动物表达系统(如CHO细胞)重组表达人源GYPB蛋白的方法。通过优化转染条件和糖基化修饰，获得具有天然构象的GYPB蛋白，验证了其在红细胞膜抗原模拟中的应用潜力。
---
2. **文献名称**: *"Structural Analysis of Recombinant GYPB for Blood Group Antigen Studies"*
**作者**: Lee J, et al.
**摘要**: 通过基因工程技术在大肠杆菌中表达可溶性GYPB胞外段重组蛋白，结合X射线晶体学解析其三维结构，揭示了GYPB与抗M/N血型抗体的结合位点，为血型分型试剂开发提供依据。
---
3. **文献名称**: *"Recombinant GYPB as a Tool for Malaria Invasion Research"*
**作者**: Patel R, et al.
**摘要**: 研究构建了重组GYPB蛋白，并验证其在疟原虫(如间日疟原虫)入侵红细胞过程中的作用。实验表明GYPB与疟原虫配体蛋白特异性结合，为抗疟疾药物靶点筛选提供了新方向。
---
注：以上文献为示例，实际引用时需查询真实数据库(如PubMed、Web of Science)获取具体信息。
The glycophorin B (GYPB) gene encodes a sialylated glycoprotein predominantly expressed on human erythrocyte membranes. As a member of the glycophorin family, GYPB plays a critical role in maintaining erythrocyte membrane integrity and facilitating cellular interactions. It carries the MN blood group antigens, which are clinically significant in transfusion medicine and prenatal testing. GYPB shares structural homology with glycophorin A (GPA), both being type I transmembrane proteins with extracellular domains rich in O-glycosylation sites. However, GYPB is less abundant than GPA and exhibits genetic polymorphisms, contributing to blood group diversity.
Recombinant GYPB protein is typically produced using heterologous expression systems, such as mammalian cells or bacteria, to study its biological functions or develop diagnostic/therapeutic tools. Its recombinant form retains key antigenic epitopes, making it valuable for generating monoclonal antibodies, analyzing blood group specificity, or investigating pathogen-host interactions (e.g., malaria parasites that exploit glycophorins for erythrocyte invasion). Additionally, recombinant GYPB serves as a critical reagent in resolving blood typing discrepancies and understanding genetic recombination events between GYPA and GYPB, which occur frequently due to their close chromosomal proximity on 4q31.21. Recent research also explores its potential role in autoimmune disorders and as a biomarker for certain hematological conditions. The development of recombinant GYPB has significantly advanced hematological research while minimizing reliance on human blood-derived materials.
×
