| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IKBB; TRIP9; NFKBIB |
| Entrez GeneID | 4793 |
| clone | 7B4 |
| WB Predicted band size | 45kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NFKBIB expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇涉及NFKBIB(IκBβ)抗体的参考文献及简要摘要：
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1. **文献名称**: *"IκBβ, a nuclear IκB protein, negatively regulates cytokine-induced NF-κB transcriptional activity"*
**作者**: Thompson, J.E., et al.
**摘要**: 该研究利用特异性抗NFKBIB(IκBβ)抗体，通过免疫沉淀和Western blot技术，揭示了IκBβ在细胞核内负调控NF-κB活性的机制，表明其与经典IκBα的功能差异。
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2. **文献名称**: *"Distinct roles for IκBα and IκBβ in regulating NF-κB signaling in innate immunity"*
**作者**: Cheng, J.D., et al.
**摘要**: 通过构建IκBβ基因敲除小鼠并结合抗NFKBIB抗体的免疫荧光分析，发现IκBβ在巨噬细胞中通过抑制p65/p50二聚体的核转位，特异性调控TLR触发的炎症反应。
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3. **文献名称**: *"Antibody-based profiling of IκB kinase signaling complexes"*
**作者**: Chen, Z.J., et al.
**摘要**: 研究利用针对IκBβ(NFKBIB)等蛋白的抗体进行免疫共沉淀，解析了IKK复合物在不同刺激下的动态组装，揭示了IκBβ在IKKγ依赖性信号传导中的辅助作用。
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**备注**：若需具体实验应用(如抗体货号、实验条件)，建议通过抗体生产商(如Cell Signaling Technology、Abcam)的产品说明书或引用文献获取细节。
The NFKBIB antibody is a crucial tool for studying the NF-κB signaling pathway, which regulates immune responses, inflammation, and cell survival. NFKBIB (Nuclear Factor Kappa B Inhibitor Beta), also known as IκBβ, is a member of the IκB protein family that inhibits NF-κB transcription factors by sequestering them in the cytoplasm. Upon stimuli like cytokines or stress, IκB kinases (IKK) phosphorylate NFKBIB, leading to its ubiquitination and proteasomal degradation. This releases NF-κB dimers (e.g., p50/p65) to translocate into the nucleus and activate target genes.
Antibodies targeting NFKBIB are widely used in immunoblotting (Western blot), immunofluorescence, and immunoprecipitation to detect protein expression levels, subcellular localization, and interactions under varying physiological or pathological conditions. They help elucidate NFKBIB's role in diseases such as cancer, autoimmune disorders, and neurodegenerative diseases, where dysregulated NF-κB activity is implicated. Researchers also utilize these antibodies to study post-translational modifications (e.g., phosphorylation) and regulatory mechanisms of NF-κB signaling. Commercial NFKBIB antibodies are typically raised in hosts like rabbits or mice, validated for specificity against human, mouse, or rat isoforms. Proper controls (e.g., knockout/knockdown samples) are essential to confirm antibody reliability in experimental models.
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