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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSH2 |
| Uniprot No | P0DML3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-217aa |
| 氨基酸序列 | VQTVPLSRLFDHAMLQAHRAHQLAIDTYQEFEETYIPKDQKYSFLHDSQTSFCFSDSIPTPSNMEETQQKSNLELLRISLLLIESWLEPVRFLRSMFANNLVYDTSDSDDYHLLKDLEEGIQTLMGRLEDGSRRTGQILKQTYSKFDTNSHNHDALLKNYGLLYCFRKDMDKVETFLRMVQCRSVEGSCGF |
| 预测分子量 | 53.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSH2(绒毛膜促生长催乳素2)重组蛋白研究的模拟参考文献示例，供参考：
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1. **文献名称**：Expression and Purification of Recombinant Human Chorionic Somatomammotropin (CSH2) in Mammalian Cells
**作者**：Lacroix MC, et al.
**摘要**：本研究报道了在CHO(中国仓鼠卵巢)细胞中成功表达重组人CSH2蛋白，通过优化载体构建和培养条件提高表达效率。纯化后的蛋白经Western blot和质谱验证，并证实其具有促进乳腺上皮细胞增殖的生物学活性。
2. **文献名称**：Functional Characterization of Recombinant CSH2 in Placental Development
**作者**：Handwerger S, et al.
**摘要**：研究利用大肠杆菌表达系统制备重组CSH2蛋白，通过体外实验证明其可激活JAK-STAT信号通路，并调控胎盘滋养层细胞的代谢功能，提示CSH2在妊娠期胎盘发育中的关键作用。
3. **文献名称**：Development of a CSH2-Specific Immunoassay Using Recombinant Protein as Antigen
**作者**：Fujimoto K, et al.
**摘要**：通过昆虫细胞(Sf9)表达重组CSH2.并以其作为抗原开发高灵敏度的ELISA检测方法，用于妊娠期妇女血清中CSH2水平的定量分析，为临床监测胎盘功能提供新工具。
4. **文献名称**：Structural Insights into Recombinant CSH2 by Circular Dichroism and X-ray Crystallography
**作者**：Chen Y, et al.
**摘要**：解析重组CSH2蛋白的晶体结构，结合圆二色光谱分析其二级结构特征，揭示其与生长激素受体的结合模式，为设计CSH2类似物奠定结构基础。
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**说明**：以上文献为模拟示例，实际研究中请通过PubMed、Web of Science等数据库检索真实发表的论文。建议以 **"chorionic somatomammotropin"** 或 **"placental lactogen"** 为关键词，结合 **"recombinant expression"** 或 **"protein purification"** 等术语进行精准检索。
CSH2 (Chorionic Somatomammotropin Hormone 2), also known as placental lactogen, is a hormone predominantly produced by the placenta during pregnancy. It belongs to the growth hormone (GH) and prolactin (PRL) family, sharing structural and functional similarities with these hormones. CSH2 plays a critical role in regulating maternal metabolism and fetal development. It promotes nutrient mobilization, stimulates insulin secretion, and modulates lipid metabolism to ensure adequate energy supply for both the mother and the growing fetus. Additionally, it contributes to mammary gland development in preparation for lactation.
Recombinant CSH2 protein is engineered using genetic engineering techniques, typically expressed in bacterial, yeast, or mammalian cell systems to ensure proper post-translational modifications and bioactivity. Its production enables researchers to study its physiological mechanisms, receptor interactions (primarily through the prolactin receptor), and potential therapeutic applications. For instance, recombinant CSH2 has been explored in studies related to gestational diabetes, intrauterine growth restriction, and lactation disorders. It also serves as a tool to investigate placental function and pregnancy-associated pathologies.
Despite its significance, CSH2's exact molecular pathways and clinical utility remain under investigation. Advances in recombinant technology have improved its stability and bioavailability, facilitating both basic research and drug development. Current efforts focus on understanding its role in maternal-fetal crosstalk and its potential as a biomarker or therapeutic agent in pregnancy complications. As a naturally occurring hormone with multifunctional properties, recombinant CSH2 holds promise for addressing unmet medical needs in obstetrics and reproductive health.
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