| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | cry1Ia |
| Uniprot No | Q45752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-719aa |
| 氨基酸序列 | MKLKNQDKHQSFSSNAKVDKISTDSLKNETDIELQNINHEDCLKMSEYENVEPFVSASTIQTGIGIAGKILGTLGVPFAGQVASLYSFILGELWPKGKNQWEIFMEHVEEIINQKISTYARNKALTDLKGLGDALAVYHDSLESWVGNRNNTRARSVVKSQYIALELMFVQKLPSFAVSGEEVPLLPIYAQAANLHLLLLRDASIFGKEWGLSSSEISTFYNRQVERAGDYSDHCVKWYSTGLNNLRGTNAESWVRYNQFRRDMTLMVLDLVALFPSYDTQMYPIKTTAQLTREVYTDAIGTVHPHPSFTSTTWYNNNAPSFSAIEAAVVRNPHLLDFLEQVTIYSLLSRWSNTQYMNMWGGHKLEFRTIGGTLNISTQGSTNTSINPVTLPFTSRDVYRTESLAGLNLFLTQPVNGVPRVDFHWKFVTHPIASDNFYYPGYAGIGTQLQDSENELPPEATGQPNYESYSHRLSHIGLISASHVKALVYSWTHRSADRTNTIEPNSITQIPLVKAFNLSSGAAVVRGPGFTGGDILRRTNTGTFGDIRVNINPPFAQRYRVRIRYASTTDLQFHTSINGKAINQGNFSATMNRGEDLDYKTFRTVGFTTPFSFLDVQSTFTIGAWNFSSGNEVYIDRIEFVPVEVTYEAEYDFEKAQEKVTALFTSTNPRGLKTDVKDYHIDQVSNLVESLSDEFYLDEKRELFEIVKYAKQLHIERNM |
| 预测分子量 | 81,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Cry1Ia重组蛋白的典型文献示例(注：文献信息为模拟概括，非真实存在)：
1. **文献名称**：*"Structural and functional analysis of Cry1Ia toxin from Bacillus thuringiensis"*
**作者**：Zhang, Y. et al.
**摘要**：通过X射线晶体学解析Cry1Ia蛋白的三维结构，发现其结构域III中的特定氨基酸残基对结合昆虫中肠受体至关重要，并通过点突变实验验证了该区域在杀虫活性中的作用。
2. **文献名称**：*"Heterologous expression of cry1Ia gene in Escherichia coli and insecticidal activity against lepidopteran pests"*
**作者**：Liu, X. et al.
**摘要**：研究利用大肠杆菌表达系统高效表达Cry1Ia重组蛋白，纯化后生物测定显示其对玉米螟(Ostrinia furnacalis)和棉铃虫(Helicoverpa armigera)幼虫具有显著致死效果，LC50值分别为12 μg/mL和18 μg/mL。
3. **文献名称**：*"Synergistic effect of Cry1Ia and Vip3Aa proteins on resistance management in Spodoptera frugiperda"*
**作者**：Wang, L. et al.
**摘要**：探讨Cry1Ia与Vip3Aa蛋白的协同作用，发现两者联合使用可延缓草地贪夜蛾对Cry1Ia的抗性进化，并通过中肠组织病理学分析揭示协同增效的分子机制。
(注：以上文献为示例性内容，实际引用需检索PubMed、Web of Science等数据库获取真实文献。)
**Background of Cry1Ia Recombinant Protein**
The Cry1Ia protein is a delta-endotoxin derived from *Bacillus thuringiensis* (Bt), a gram-positive soil bacterium widely used in biocontrol due to its insecticidal properties. Cry proteins, including Cry1Ia, are toxic to specific insect larvae, particularly Lepidoptera (e.g., moths and butterflies), by forming pores in the midgut epithelium, leading to osmotic imbalance and larval death. Cry1Ia belongs to the three-domain Cry1 family but exhibits distinct structural features that influence its target specificity and binding efficiency to insect receptors.
Recombinant Cry1Ia is produced through genetic engineering, where the *cry1Ia* gene is cloned into expression systems like *Escherichia coli* or yeast. This allows scalable production, purification, and modification to enhance stability or toxicity. Unlike native Bt formulations, recombinant Cry1Ia can be tailored for broader pest resistance management, addressing issues like field-evolved insect resistance.
Research on Cry1Ia focuses on optimizing its expression, elucidating its mode of action, and integrating it into transgenic crops (e.g., Bt cotton or maize) or biopesticides. Its environmental safety and non-target organism impacts are also critical study areas. Recent advances include hybrid Cry proteins combining Cry1Ia domains with other Bt toxins to expand insecticidal spectra and delay resistance.
Overall, Cry1Ia represents a sustainable tool in integrated pest management, reducing reliance on chemical pesticides while aligning with agricultural sustainability goals.
×
