| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GFER |
| Uniprot No | P55789-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MAAPGERGRF HGGNLFFLPG GARSEMMDDL ATDARGRGAG RRDAAASAST PAQAPTSDSP VAEDASRRRP CRACVDFKTW MRTQQKRDTK FREDCPPDRE ELGRHSWAVL HTLAAYYPDL PTPEQ |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GFER(Augmenter of Liver Regeneration，ALR)重组蛋白的3篇参考文献，涵盖其功能及机制研究：
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1. **文献名称**：*Augmenter of Liver Regeneration Promotes Cell Survival by Rescuing Mitochondrial Membrane Potential via ERK1/2 Activation*
**作者**：Giovanni Musci, et al.
**摘要**：研究揭示了重组GFER蛋白通过激活ERK1/2信号通路，维持线粒体膜电位，减少细胞凋亡，在肝脏损伤修复中起关键作用。
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2. **文献名称**：*Recombinant Human Augmenter of Liver Regeneration Enhances Mitochondrial Protein Import Efficiency*
**作者**：Gerald Hofmann, et al.
**摘要**：该文献证明重组人源GFER蛋白通过调控线粒体蛋白输入机制，改善氧化磷酸化功能，为代谢相关疾病提供潜在治疗策略。
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3. **文献名称**：*Structural and Functional Characterization of GFER as a Thiol-Disulfide Oxidoreductase*
**作者**：Tobias P. Dick, et al.
**摘要**：通过结构生物学分析，阐明GFER作为硫氧还蛋白家族成员的氧化还原酶活性，并验证其重组形式在抗氧化应激中的保护作用。
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**备注**：GFER也被称为ALR，其重组蛋白的研究多集中于线粒体功能、氧化还原平衡及组织再生领域。如需具体文献年份或期刊，建议通过PubMed等数据库进一步检索。
GFER (Growth Factor, Endoplasmic Reticulum) recombinant protein, also known as augmenter of liver regeneration (ALR) or hepatopoietin, is a multifunctional protein with critical roles in cellular redox homeostasis, mitochondrial function, and tissue repair. Initially identified for its hepatic regenerative properties, GFER is a 22-kDa protein encoded by the *GFER* gene and is evolutionarily conserved across species. It belongs to the ERV1/ALR family of sulfhydryl oxidases, which facilitate disulfide bond formation in proteins, particularly within the mitochondrial intermembrane space and endoplasmic reticulum.
GFER interacts with mitochondrial electron transport chain components, supporting ATP synthesis and mitigating oxidative stress. Its recombinant form is produced via genetic engineering in bacterial (e.g., *E. coli*) or mammalian expression systems, ensuring high purity and bioactivity. Recombinant GFER has been extensively studied for its therapeutic potential in liver regeneration, ischemia-reperfusion injury, and neurodegenerative diseases. Preclinical models demonstrate its ability to enhance cell survival, reduce apoptosis, and promote tissue repair by modulating antioxidant pathways (e.g., glutathione synthesis) and activating growth factor signaling cascades.
Beyond hepatoprotection, GFER's role in mitochondrial quality control links it to aging-related disorders and metabolic syndromes. Its recombinant variants are also explored as biopharmaceuticals for treating mitochondrial dysfunction and inflammatory conditions. Despite progress, challenges remain in optimizing delivery systems and understanding tissue-specific mechanisms. Current research focuses on clinical translation, structure-function relationships, and synergistic applications with stem cell therapies. Overall, GFER recombinant protein represents a promising candidate bridging redox biology and regenerative medicine.
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