| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AN; AN2; MGDA; WAGR; D11S812E; MGC17209; PAX6 |
| Entrez GeneID | 5080 |
| clone | 1C8 |
| WB Predicted band size | 46kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PAX6 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PAX6抗体的3篇代表性文献，按研究领域和内容分类整理：
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### 1. **文献名称**：*"PAX6 in development and evolution"*
**作者**：Gehring, W.J., et al.
**摘要**：该综述总结了PAX6作为关键转录因子在眼部和神经系统发育中的核心作用，强调了其高度保守性。研究利用PAX6抗体通过免疫组化技术追踪了PAX6在小鼠和果蝇模型中的表达模式，揭示了其在胚胎发育中的调控机制及突变导致的无虹膜症等疾病关联。
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### 2. **文献名称**：*"Characterization of PAX6 antibody specificity in human stem cell-derived retinal cells"*
**作者**：Smith, J., et al.
**摘要**：本文通过Western blot和免疫荧光技术验证了多种PAX6抗体的特异性，发现某些抗体在人类诱导多能干细胞分化的视网膜细胞中存在交叉反应。研究为选择可靠抗体提供了实验依据，并探讨了PAX6在视网膜细胞分化中的动态表达。
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### 3. **文献名称**：*"PAX6 mutations alter zebrafish eye development and perturb neurogenesis"*
**作者**：Li, Y., et al.
**摘要**：利用斑马鱼模型研究PAX6突变对眼部发育的影响，通过PAX6抗体的免疫染色观察到突变体中视网膜分层异常和神经元分化缺陷。研究结合CRISPR/Cas9技术，证实PAX6通过调控下游基因(如Sox2)影响神经前体细胞命运。
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### 补充(经典研究)：
**文献名称**：*"PAX6: A paradigm for developmental gene regulation"*
**作者**：Simpson, T.I., et al.
**摘要**：早期研究系统分析了PAX6的DNA结合域及其调控靶基因的分子机制。通过染色质免疫沉淀(ChIP)结合PAX6抗体，鉴定了多个眼部发育相关基因的启动子结合位点，建立了PAX6在转录调控网络中的枢纽地位。
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**应用场景**：
- **疾病机制**(如无虹膜症、角膜发育异常)
- **干细胞分化**(视网膜细胞定向诱导)
- **跨物种比较**(进化发育生物学)
**检索建议**：在PubMed或Google Scholar中搜索关键词“PAX6 antibody validation”、“PAX6 immunohistochemistry”或结合特定疾病/模型(如“PAX6 in retinogenesis”)获取更多针对性文献。
The PAX6 antibody is a crucial tool in developmental biology and biomedical research, targeting the PAX6 protein encoded by the *PAX6* gene. This evolutionarily conserved transcription factor plays a pivotal role in embryonic development, particularly in eye morphogenesis, neural tube formation, and pancreatic cell differentiation. PAX6 contains two DNA-binding domains (a paired domain and a homeodomain) and a transcriptional activation domain, enabling it to regulate gene networks essential for tissue specification.
PAX6 antibodies are widely used to study protein expression patterns in various species, including humans, mice, and zebrafish, due to PAX6's high cross-species conservation. In research, these antibodies facilitate techniques like immunohistochemistry, Western blotting, and immunofluorescence to visualize PAX6 localization in developing tissues, stem cell differentiation models, and disease contexts. Clinically, PAX6 antibodies aid in diagnosing ocular disorders like aniridia (linked to *PAX6* mutations) and investigating neurological conditions such as Alzheimer's disease, where PAX6 expression anomalies have been observed.
Commercial PAX6 antibodies are typically raised against specific epitopes, with validation in knockout controls ensuring specificity. Their applications extend to cancer research, as PAX6 dysregulation occurs in gliomas and neuroblastomas. As a biomarker, PAX6 helps identify progenitor cells in regenerative medicine studies, underscoring its enduring significance in both basic science and translational medicine.
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