Mouse Monoclonal CDC27 Antibody

The CDC27 antibody targets the CDC27 protein, a key component of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase critical for cell cycle regulation. CDC27. also known as APC3. plays a central role in mediating the transition from metaphase to anaphase by tagging specific cell cycle regulators (e.g., securin, cyclin B) with ubiquitin for proteasomal degradation. This process ensures proper mitotic exit and genomic stability. Dysregulation of APC/C function, including CDC27. is linked to mitotic errors, chromosomal instability, and diseases such as cancer. CDC27 antibodies are widely used in research to study cell cycle dynamics, APC/C assembly, and protein interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. They also help assess CDC27 expression levels in pathological contexts, providing insights into tumor progression or therapeutic responses. As a conserved protein across eukaryotes, CDC27 antibodies may cross-react with homologs in model organisms, facilitating comparative studies. However, specificity validation (e.g., knockout controls) is essential due to potential cross-reactivity with related APC/C subunits. Overall, CDC27 antibodies serve as vital tools for unraveling mechanisms of cell division and their implications in health and disease.