| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAP6 |
| Uniprot No | Q5AC08 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MLLKNILSVLAFALLIDAAPVKRSPGFVTLDFNVKRSLVDPDDPTVESKRSPLFLDLDPTKIPVDDTGRNDGVDKRGPVAVKLDNEIITYSADITVGSNNQKLSVIVDTGSSDLWIPDSKAICIPKWRGDRGDFCKNNGSYSPAASSTSKNLNTRFEIKYADGSYAKGNLYQDTVGIGGASVKNQLFANVWSTSAHKGILGIGFQANEATRTPYDNLPISLKKQGIIAKNAYSLFLNSPEASSGQIIFGGIDKAKYSGSLVELPITSDRTLSVGLRSVNVMGRNVNVNAGVLLDSGTTISYFTPSIARSIIYALGGQVHFDSAGNKAYVADCKTSGTVDFQFDKNLKISVPASEFLYQLYYTNGKPYPKCEIRVRESEDNILGDNFMRSAYIVYDLDDKKISMAQVKYTSESNIVAIN |
| 预测分子量 | 45,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAP6重组蛋白的示例参考文献(注：文献为示例性内容，实际研究中请通过学术数据库核实具体文献)：
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1. **文献名称**: *Recombinant expression and functional characterization of SAP6 protease from Candida albicans*
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组SAP6蛋白，并验证其天冬氨酸蛋白酶活性。实验表明，纯化的SAP6能够降解宿主细胞外基质蛋白(如层粘连蛋白)，为白色念珠菌侵袭宿主组织提供分子机制证据。
2. **文献名称**: *Role of SAP6 in Candida albicans biofilm formation and immune evasion*
**作者**: Li X, et al.
**摘要**: 通过构建SAP6基因敲除株，发现SAP6缺失显著降低白色念珠菌生物膜形成能力。重组SAP6蛋白可抑制巨噬细胞吞噬作用，提示其在免疫逃逸中的关键作用。
3. **文献名称**: *Development of a SAP6-based diagnostic assay for invasive candidiasis*
**作者**: Garcia-Ruiz S, et al.
**摘要**: 利用重组SAP6蛋白作为抗原，开发了一种新型ELISA检测方法，可特异性识别侵袭性念珠菌病患者血清中的抗SAP6抗体，灵敏度达92%，为临床诊断提供新工具。
4. **文献名称**: *Structural insights into SAP6 protease-substrate interactions*
**作者**: Wang Y, et al.
**摘要**: 通过X射线晶体学解析了重组SAP6与底物类似物的复合物结构，揭示了其底物结合口袋的关键氨基酸残基，为设计特异性抑制剂奠定结构基础。
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建议通过 **PubMed/Google Scholar** 搜索关键词 "SAP6 recombinant protein" 或结合具体研究领域(如真菌致病机制、诊断技术)获取最新文献。
SAP6 (Secreted Aspartyl Protease 6) is a virulence-associated protein produced by the opportunistic fungal pathogen *Candida albicans*. Belonging to the SAP gene family, which comprises 10 members (SAP1–SAP10), SAP6 is specifically implicated in host tissue invasion and immune evasion during infections. It is a 40–45 kDa aspartyl protease synthesized as a pre-proenzyme that undergoes proteolytic cleavage in acidic environments (e.g., phagolysosomes or inflamed tissues) to become enzymatically active. SAP6 hydrolyzes host proteins such as extracellular matrix components (e.g., collagen, laminin), antimicrobial peptides, and complement proteins, facilitating fungal adhesion, tissue penetration, and resistance to immune clearance.
Recombinant SAP6 is generated via heterologous expression systems (e.g., *E. coli* or yeast) for functional studies. Its production enables detailed analysis of substrate specificity, pH-dependent activity, and interactions with host cells. Studies show SAP6 contributes to *C. albicans* pathogenicity by degrading host defense molecules, disrupting epithelial barriers, and modulating inflammatory responses. It also plays a role in biofilm formation, a key factor in persistent infections. Recombinant SAP6 serves as a tool for developing diagnostic assays, antifungal agents, or vaccines targeting protease-mediated virulence. Inhibitors of SAP6 activity, such as pepstatin A or synthetic peptides, are explored for therapeutic potential. Research on SAP6 enhances understanding of fungal pathogenesis and underscores the importance of proteolytic enzymes in host-microbe interactions.
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