| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Defb19 |
| Uniprot No | Q8N690 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-84aa |
| 氨基酸序列 | KRHILRCMG NSGICRASCK KNEQPYLYCR NCQSCCLQSY MRISISGKEE NTDWSYEKQW PRLP |
| 预测分子量 | 9,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于Defb19(Defensin beta 19)重组蛋白的研究目前公开报道较少，以下为模拟的文献示例(实际文献需通过PubMed或SciFinder等数据库检索)：
1. **标题**：Recombinant Defb19 exhibits antimicrobial activity against Gram-negative bacteria
**作者**：Smith A, et al.
**摘要**：研究成功在大肠杆菌中表达并纯化重组Defb19蛋白，证明其对大肠杆菌和铜绿假单胞菌具有剂量依赖性抑菌作用，可能通过破坏细菌膜结构发挥作用。
2. **标题**：Defb19 regulates intestinal epithelial barrier function
**作者**：Chen L, et al.
**摘要**：发现重组Defb19可通过激活TLR4/NF-κB信号通路增强肠道上皮细胞紧密连接蛋白表达，改善小鼠结肠炎模型的肠屏障损伤。
3. **标题**：Expression optimization of human Defb19 in Pichia pastoris
**作者**：Wang Y, et al.
**摘要**：优化毕赤酵母表达系统实现Defb19的高效分泌表达，通过亲和层析获得高纯度蛋白，为后续功能研究提供基础。
**说明**：以上内容为假设性示例，Defensin家族蛋白的实际研究多集中于Defb1、Defb4等成员。建议通过以下途径获取真实文献：
1. 检索关键词："Defensin beta 19 recombinant"或"DEFB19 protein"
2. 筛选涉及重组表达、抗菌机制或疾病模型的论文
3. 关注《Antimicrobial Agents and Chemotherapy》《Biochemical Journal》等期刊的相关研究
Defensin beta 19 (DEFB19), a member of the β-defensin family, is a small cationic antimicrobial peptide (AMP) encoded by the DEFB19 gene in humans. β-defensins are evolutionarily conserved innate immune molecules broadly expressed in epithelial and mucosal tissues, where they play critical roles in host defense against pathogens, immune modulation, and tissue homeostasis. DEFB19 is primarily produced in reproductive tissues, such as the epididymis and testes, suggesting potential roles in antimicrobial protection of the male reproductive tract and sperm function. Structurally, it features a conserved six-cysteine motif forming three disulfide bonds, which stabilizes its β-sheet-rich fold essential for microbial membrane disruption.
Recombinant DEFB19 protein is engineered using biotechnological platforms (e.g., bacterial or mammalian expression systems) to produce functional peptides for research and therapeutic exploration. Its recombinant form enables studies on mechanisms of antimicrobial activity, including broad-spectrum antibacterial, antiviral, and antifungal effects. Additionally, DEFB19 has been implicated in chemotaxis, wound healing, and cancer cell regulation, expanding its potential biomedical applications. Challenges in recombinant production include maintaining proper folding and post-translational modifications critical for bioactivity. Current research focuses on optimizing expression systems, elucidating structure-function relationships, and evaluating DEFB19’s therapeutic potential in infections, infertility, and inflammatory diseases. Despite limited clinical data, DEFB19 represents a promising candidate for developing novel antimicrobial agents and immunomodulators.
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