| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC7A5 |
| Uniprot No | Q01650 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-50aa |
| 氨基酸序列 | AEEKEEAREKMLAAKSADGSAPAGEGEGVTLQRNI |
| 预测分子量 | 18.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SLC7A5重组蛋白相关的研究文献摘要(基于近年研究整理)：
1. **文献名称**：*Structural basis of amino acid recognition by the SLC7A5/LAT1 transporter*
**作者**：Lee Y. et al. (2022)
**摘要**：通过重组表达人源SLC7A5蛋白并进行冷冻电镜解析，揭示了其识别大中性氨基酸(如亮氨酸)的分子机制，为靶向该蛋白的癌症治疗药物设计提供结构基础。
2. **文献名称**：*SLC7A5-mediated glutamine transport promotes tumor growth in pancreatic cancer*
**作者**：Wang Q. et al. (2021)
**摘要**：利用重组SLC7A5蛋白体外模型，证明其在胰腺癌细胞中对谷氨酰胺转运的关键作用，并发现抑制SLC7A5可显著减缓肿瘤生长。
3. **文献名称**：*Recombinant SLC7A5 protein enables targeted drug delivery across the blood-brain barrier*
**作者**：Chen L. et al. (2020)
**摘要**：研究团队构建了SLC7A5重组蛋白脂质体载体，验证其通过血脑屏障的氨基酸转运机制，为中枢神经系统药物的靶向递送提供新策略。
(注：以上文献信息为模拟示例，实际引用需以具体论文数据为准。)
**Background of SLC7A5 Recombinant Protein**
SLC7A5. also known as LAT-1 (L-type amino acid transporter 1), is a member of the solute carrier family 7 (SLC7), which plays a critical role in cellular nutrient transport. It is a transmembrane protein that forms a heterodimeric complex with CD98 (SLC3A2) to function as a sodium-independent neutral amino acid transporter. This transporter primarily facilitates the uptake of essential branched-chain and aromatic amino acids, such as leucine, phenylalanine, and tryptophan, which are vital for protein synthesis, cell growth, and mTOR signaling pathway activation.
SLC7A5 is highly expressed in tissues with high metabolic demands, including the blood-brain barrier (BBB), placenta, and certain cancers. Its overexpression in tumors supports cancer progression by supplying amino acids necessary for rapid proliferation and immune evasion. This has positioned SLC7A5 as a potential therapeutic target in oncology.
Recombinant SLC7A5 protein is engineered using expression systems like mammalian cells or bacteria to produce purified, functional forms of the protein for research. It enables studies on transport mechanisms, substrate specificity, and interactions with inhibitors or drugs. Researchers also utilize it to develop assays for screening compounds that modulate its activity, aiming to discover therapies that disrupt cancer metabolism or enhance drug delivery across the BBB.
Despite its promise, challenges remain in understanding its regulation and tissue-specific roles. Ongoing research focuses on elucidating its structural biology and validating its therapeutic potential while minimizing off-target effects in healthy tissues.
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