| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GF1; GF-1; NFE1; XLTT; ERYF1; GATA-1; GATA1 |
| Entrez GeneID | 2623 |
| clone | 4G1 |
| WB Predicted band size | 42.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GATA1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇与GATA1抗体相关的代表性文献，按研究领域和内容概括：
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1. **文献名称**：*GATA-1: A transcription factor that regulates erythroid development and globin gene expression*
**作者**：Stuart H. Orkin
**摘要**：该综述总结了GATA1作为红系发育关键转录因子的功能，重点描述其通过结合DNA特定序列调控血红蛋白基因表达的机制。文中提及GATA1抗体的应用，例如通过染色质免疫沉淀(ChIP)验证其在靶基因启动子区的结合。
2. **文献名称**：*Disruption of the GATA-1 gene in human acute megakaryoblastic leukemia*
**作者**：Alice P. Tsai 等
**摘要**：研究报道GATA1基因突变与急性巨核细胞白血病(AMKL)的关联，利用GATA1特异性抗体进行免疫印迹和免疫组化分析，发现突变导致GATA1蛋白截短体表达异常，破坏正常造血分化。
3. **文献名称**：*GATA1 regulates the generation of platelets from human megakaryocytes*
**作者**：Ramesh A. Shivdasani 等
**摘要**：通过GATA1抗体阻断实验和基因敲除模型，证明GATA1在巨核细胞成熟和血小板生成中的必要性，并揭示其调控下游靶基因(如GPIIb和PF4)的分子机制。
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**扩展方向**：若需更具体的实验类文献(如抗体在流式细胞术或免疫荧光中的应用)，可进一步补充。
The GATA1 antibody is a crucial tool in studying the GATA-binding protein 1 (GATA1), a transcription factor essential for hematopoiesis. GATA1. a member of the GATA family, regulates gene expression by binding to DNA motifs containing the consensus sequence (A/T)GATA(A/G). It plays a pivotal role in erythroid and megakaryocytic differentiation, controlling the development of red blood cells and platelets. Structurally, GATA1 contains two zinc finger domains: the C-terminal zinc finger mediates DNA binding, while the N-terminal finger stabilizes interactions with cofactors like FOG1 (Friend of GATA1). Dysregulation or mutations in GATA1 are linked to blood disorders, such as Diamond-Blackfan anemia, thalassemia, and leukemia.
The GATA1 antibody enables researchers to detect and quantify GATA1 protein levels in various experimental models, including Western blotting, immunohistochemistry, and flow cytometry. It aids in investigating GATA1's role in normal hematopoiesis, its interplay with signaling pathways, and its involvement in malignancies. Commercially available GATA1 antibodies are typically raised against specific epitopes, ensuring specificity for applications in both human and murine samples. Validated antibodies are critical for studies exploring GATA1's regulatory mechanisms, such as its cooperation with other transcription factors (e.g., SCL/TAL1) or its repression of myeloid differentiation genes. Overall, GATA1 antibodies are indispensable in advancing our understanding of hematologic development, disease pathogenesis, and potential therapeutic targets.
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