| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT34 |
| Uniprot No | O76011 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-436aa |
| 氨基酸序列 | MLYAKPPPTINGIKGLQRKERLKPAHIHLQQLTCFSITCSSTMSYSCCLPSLGCRTSCSSRPCVPPSCHGYTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELEKLIQERSQQQEPLLCPSYQSYFKTIEELQQKILCAKAENARLVVNIDNAKLASDDFRSKYQTEQSLRLLVESDINSIRRILDELTLCKSDLESQVESLREELICLKKNHEEEVNTLRSQLGDRLNVEVDTAPTVDLNQVLNETRSQYEALVEINRREVEQWFATQTEELNKQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHNLRDSLENTLTESEAHYSSQLSQVQSLITNVESQLAEIRCDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPCNPCATTNASGNSCGPCGTSQKGCCN |
| 预测分子量 | 78.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT34重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库核实)：
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1. **文献名称**: *"Recombinant Expression and Characterization of Human Hair Keratin KRT34 in Escherichia coli"*
**作者**: Zhang, L. et al.
**摘要**: 本研究报道了人源KRT34基因在大肠杆菌系统中的重组表达与纯化。通过优化密码子和诱导条件，成功获得可溶性KRT34蛋白，并验证其与天然角蛋白相似的二级结构，为后续研究毛发疾病的分子机制提供工具。
2. **文献名称**: *"Functional Analysis of KRT34 Mutations Using Recombinant Proteins in a 3D Epidermal Model"*
**作者**: Gupta, R. & Patel, S.
**摘要**: 利用哺乳动物表达系统制备重组KRT34蛋白，结合CRISPR编辑技术构建突变体，研究其在3D表皮模型中与KRT85的相互作用。发现特定突变导致角蛋白网络稳定性下降，提示其在遗传性毛发疾病中的潜在作用。
3. **文献名称**: *"Production of Recombinant KRT34 for Antibody Development in Monoclonal Therapy"*
**作者**: Kim, H. et al.
**摘要**: 通过杆状病毒-昆虫细胞系统高效表达重组KRT34蛋白，经亲和层析纯化后免疫小鼠，成功制备特异性单克隆抗体。该抗体可用于诊断性免疫组化标记，为毛发相关肿瘤的生物标志物研究奠定基础。
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**备注**：实际文献需通过PubMed、Web of Science等平台检索关键词“KRT34 recombinant protein”或“recombinant keratin 34”获取。建议结合具体研究方向(如疾病机制、结构生物学)筛选文献。
Keratin 34 (KRT34) is a member of the keratin family, a group of structural proteins critical for maintaining the integrity of epithelial cells and appendages such as hair and nails. As part of the type I intermediate filament proteins, KRT34 pairs with type II keratins to form heterodimers, which assemble into robust cytoskeletal networks. These networks provide mechanical strength, regulate cell signaling, and protect against stress. KRT34 is specifically expressed in the hair cortex and cuticle layers, playing a pivotal role in hair shaft formation and resilience. Mutations in the KRT34 gene are linked to hereditary hair disorders like monilethrix, characterized by fragile, beaded hair and alopecia.
Recombinant KRT34 protein is engineered using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to produce purified, functional keratin for research and therapeutic applications. Its production enables detailed studies of keratin structure-function relationships, particularly how specific mutations disrupt filament assembly or stability. Researchers use recombinant KRT34 to model hair disorders in vitro, screen potential therapeutics, and develop gene-editing strategies. Additionally, it serves as an antigen for generating antibodies to detect keratin expression in tissues or diagnose keratin-related pathologies. Beyond hair biology, insights from KRT34 studies may inform broader understanding of intermediate filament dynamics in epithelial integrity, wound healing, and cancer. The protein’s stability and biocompatibility also spark interest in biomaterial engineering, such as keratin-based scaffolds for regenerative medicine. Overall, recombinant KRT34 bridges basic keratin biology with translational applications in dermatology and biotechnology.
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