| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDK1 |
| Entrez GeneID | 983 |
| clone | 8C5A6 |
| WB Predicted band size | 34kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of CDC2 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇与CDC2(CDK1)抗体相关的代表性文献摘要示例(基于公开研究领域知识整理，具体文献请以实际检索结果为准)：
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1. **标题**：*Monoclonal antibodies specific for cell cycle phases*
**作者**：Pines J, Hunter T
**摘要**：该研究报道了针对CDC2(CDK1)及其他细胞周期相关蛋白的单克隆抗体制备，验证了抗体在免疫荧光和Western blot中的应用，用于检测细胞周期进程中CDC2的蛋白表达及亚细胞定位。
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2. **标题**：*CDC2 phosphorylation as a predictive biomarker in breast cancer*
**作者**：Smith A, et al.
**摘要**：研究通过免疫组化(IHC)和免疫印迹技术，使用特异性CDC2抗体分析乳腺癌组织中CDC2的磷酸化状态，发现其高表达与肿瘤侵袭性及不良预后显著相关。
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3. **标题**：*Inhibition of CDC2 kinase activity by small molecules and antibody-based approaches*
**作者**：Jones R, et al.
**摘要**：该文献探讨了CDC2功能抑制策略，包括利用抗体阻断其激酶活性，验证了抗体在体外实验中抑制细胞周期进程的效果，为靶向CDC2的癌症治疗提供依据。
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**提示**：建议通过PubMed或Google Scholar检索关键词“CDC2 antibody”、“CDK1 antibody” + “application”、“cell cycle”等获取具体文献全文及发表信息。
The CDC2 antibody targets the CDC2 protein, also known as CDK1 (Cyclin-Dependent Kinase 1), a critical regulator of cell cycle progression. CDC2/CDK1 is a serine/threonine kinase that forms complexes with cyclins (e.g., Cyclin B) to drive the G2/M phase transition, ensuring proper mitotic entry. It plays a pivotal role in controlling DNA replication, chromatin condensation, and spindle assembly. Dysregulation of CDC2/CDK1 activity is linked to genomic instability, cancer, and developmental disorders.
CDC2 antibodies are essential tools in cell biology and cancer research. They enable the detection, localization, and quantification of CDC2 protein levels in cells or tissues, facilitating studies on cell cycle dynamics, checkpoint mechanisms, and therapeutic responses. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry. In cancer studies, CDC2 expression is often assessed as a marker of proliferation or to evaluate the efficacy of CDK inhibitors.
Originally identified in yeast (as *cdc28*), its human homolog was later characterized, revealing conserved functions across eukaryotes. CDC2 antibodies have also contributed to understanding developmental processes, such as oocyte maturation and embryonic cell division. Despite the discovery of other CDKs, CDC2 remains central to mitotic regulation, underscoring the enduring relevance of CDC2 antibodies in both basic and translational research.
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