| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Gsdmdc1 |
| Uniprot No | Q9D8T2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 277-487aa |
| 氨基酸序列 | GIDEEELIEAADFQGLYAEVKACSSELESLEMELRQQILVNIGKILQDQPSMEALEASLGQGLCSGGQVEPLDGPAGCILECLVLDSGELVPELAAPIFYLLGALAVLSETQQQLLAKALETTVLSKQLELVKHVLEQSTPWQEQSSVSLPTVLLGDCWDEKNPTWVLLEECGLRLQVESPQVHWEPTSLIPTSALYASLFLLSSLGQKPC |
| 预测分子量 | 30.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Gsdmdc1重组蛋白的假设性参考文献示例(注：实际文献可能需要通过学术数据库验证)：
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1. **文献名称**: *Gasdermin Dc1 triggers pyroptosis through pore-forming activity*
**作者**: Zhang Y, Chen Q, et al.
**摘要**: 研究利用重组Gsdmdc1蛋白证明其能在细胞膜上形成纳米孔隙，介导IL-1β释放和细胞焦亡，揭示了其在炎症反应中的关键作用。
2. **文献名称**: *Recombinant Gsdmdc1 expression and purification for functional analysis*
**作者**: Li S, Wang T, et al.
**摘要**: 报道了Gsdmdc1重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其诱导巨噬细胞焦亡的活性，为后续机制研究提供工具。
3. **文献名称**: *Structural insights into Gsdmdc1 activation by caspase cleavage*
**作者**: Liu X, Rao Z, et al.
**摘要**: 通过重组Gsdmdc1蛋白的晶体结构解析，阐明caspase酶切对其构象变化的调控，解释了焦亡信号通路的分子基础。
4. **文献名称**: *Gsdmdc1-mediated pyroptosis in bacterial infection models*
**作者**: Xu R, et al.
**摘要**: 使用重组Gsdmdc1蛋白回补实验，证实其在沙门氏菌感染中促进宿主细胞焦亡并限制病原体增殖的双重功能。
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**注意**：以上为模拟文献，实际研究中请通过PubMed或Google Scholar检索真实文献(关键词：Gsdmdc1 recombinant protein, pyroptosis, gasdermin family)。若研究较少，可扩展至GSDMD相关文献或检查基因命名准确性。
**Background of Gsdmdc1 Recombinant Protein**
Gsdmdc1 (Gasdermin domain-containing protein 1) is a member of the gasdermin protein family, which is evolutionarily conserved and plays critical roles in programmed cell death pathways, particularly pyroptosis. Gasdermins are characterized by a conserved N-terminal pore-forming domain and a C-terminal autoinhibitory domain. Under physiological or pathological conditions, proteolytic cleavage (often mediated by caspases or granzymes) releases the N-terminal fragment, which oligomerizes to form pores in the cell membrane, leading to lytic cell death and inflammatory cytokine release.
Gsdmdc1 shares structural homology with other gasdermin members, such as GSDMD and GSDME, but exhibits distinct tissue expression patterns and regulatory mechanisms. While its precise biological functions remain less characterized compared to other gasdermins, emerging evidence suggests its involvement in immune responses, cancer progression, and tissue homeostasis. For instance, Gsdmdc1 has been implicated in modulating inflammation in certain cancers, where its dysregulation may contribute to tumorigenesis or antitumor immunity.
Recombinant Gsdmdc1 protein is engineered to study its biochemical properties, interactions, and functional roles *in vitro* or *in vivo*. This tool enables researchers to investigate cleavage kinetics, pore-forming activity, and downstream signaling pathways. Additionally, it aids in exploring therapeutic potential, such as targeting Gsdmdc1 in inflammatory diseases or cancer immunotherapy. Challenges remain in fully elucidating its activation triggers, tissue-specific roles, and crosstalk with other cell death pathways, highlighting the need for further research using recombinant protein-based approaches.
Overall, Gsdmdc1 represents a promising yet understudied player in cell death and immune regulation, with recombinant proteins serving as vital reagents to unravel its pathophysiological significance.
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