| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDI1 |
| Uniprot No | P31150 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-447aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGS MDEEYDV IVLGTGLTEC ILSGIMSVNG KKVLH MDRNP YYGGESSSIT PLEELYKRFQ LLEGPPESMG RGRDWNVDLI PKFLMANGQL VKMLLYTEVT RYLDFKVVEG SFVYKGGKIY KVPSTETEAL ASNLMGMFEK RRFRKFLVFV ANFDENDPKT FEGVDPQTTS MRDVYRKFDL GQDVIDFTGH ALALYRTDDY LDQPCLETVN RIKLYSESLA RYGKSPYLYP LYGLGELPQG FARLSAIYGG TYMLNKPVDD IIMENGK VVG VKSEGEVARC KQLICDPSYI PDRVRKAGQV IRIICILSHP IKNTNDANSC QIIIPQNQVN RKSDIYVCMI SYAHNVAAQG KYIAIASTTV ETTDPEKEVE PALELLEPID QKFVAISDLY EPIDDGCESQ VFCSCSYDAT THFETTCNDI KDIYKRMAGT AFDFENMKRK QNDVFGEAEQ |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GDI1重组蛋白研究的示例参考文献(注：以下文献为示例性描述，实际文献需通过学术数据库检索验证):
---
1. **文献名称**: "Expression and Purification of Recombinant GDI1 in Escherichia coli for Functional Studies"
**作者**: Smith J, et al.
**摘要**: 报道了利用大肠杆菌表达系统高效表达人源GDI1重组蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其与Rab GTPases的结合活性。
2. **文献名称**: "Structural Insights into GDI1-Mediated Membrane Extraction of Rab Proteins"
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组GDI1蛋白与Rab3A的复合物结构，揭示了GDI1通过疏水口袋捕获Rab蛋白的分子机制，为神经递质释放调控提供结构基础。
3. **文献名称**: "GDI1 Dysfunction in X-linked Intellectual Disability: Recombinant Protein Rescue in Cellular Models"
**作者**: Chen R, et al.
**摘要**: 构建了突变型GDI1重组蛋白，证明其无法有效回收Rab蛋白至细胞膜，并利用野生型重组GDI1恢复患者来源神经细胞的囊泡运输缺陷。
4. **文献名称**: "A Novel GDI1 Recombinant Fusion Protein Enhances Drug Delivery Efficiency in vitro"
**作者**: Wang Y, et al.
**摘要**: 设计了一种GDI1与穿膜肽融合的重组蛋白，证实其可通过调控囊泡运输显著提高靶向药物在肿瘤细胞内的递送效率。
---
**注意**: 实际研究中建议通过PubMed、Web of Science等平台，以关键词“GDI1 recombinant”或“GDI1 purification”检索最新文献。部分GDI1研究可能侧重于其基因突变与疾病(如X-linked智力障碍)的关联，重组蛋白相关文献需进一步筛选。
**Background of GDI1 Recombinant Protein**
GDI1 (GDP Dissociation Inhibitor 1) is a regulatory protein involved in intracellular vesicle trafficking and membrane dynamics. It belongs to the RabGDI family, which modulates the activity of Rab GTPases—key regulators of membrane transport, organelle identity, and vesicle fusion. Specifically, GDI1 binds to Rab proteins in their GDP-bound inactive state, maintaining their solubility in the cytoplasm and recycling them from membranes for reuse in subsequent trafficking cycles. This function is critical for maintaining cellular homeostasis, neurotransmitter release, and receptor trafficking in neurons.
The *GDI1* gene is located on the X chromosome (Xq28) and has been linked to X-linked intellectual disability (XLID). Mutations in GDI1 disrupt Rab protein cycling, impair synaptic vesicle recycling, and lead to neuronal dysfunction, underscoring its importance in neurodevelopment.
Recombinant GDI1 protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. It is purified for use in biochemical assays, structural studies, and functional experiments to investigate Rab-GDI interactions, vesicle transport mechanisms, and disease pathophysiology. Researchers also employ recombinant GDI1 to explore therapeutic strategies for neurological disorders linked to GDI1 dysfunction, such as gene therapy or small-molecule interventions to restore Rab cycling.
Its applications extend to drug discovery, diagnostic tool development, and cellular pathway modeling, making it a valuable tool in both basic research and translational medicine.
×
