| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZC3H7B |
| Uniprot No | Q9UGR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 481-915aa |
| 氨基酸序列 | SYYLCKDMINKQDCKYGDNCTFAYHQEEIDVWTEERKGTLNRDLLFDPLGGVKRGSLTIAKLLKEHQGIFTFLCEICFDSKPRIISKGTKDSPSVCSNLAAKHSFYNNKCLVHIVRSTSLKYSKIRQFQEHFQFDVCRHEVRYGCLREDSCHFAHSFIELKVWLLQQYSGMTHEDIVQESKKYWQQMEAHAGKASSSMGAPRTHGPSTFDLQMKFVCGQCWRNGQVVEPDKDLKYCSAKARHCWTKERRVLLVMSKAKRKWVSVRPLPSIRNFPQQYDLCIHAQNGRKCQYVGNCSFAHSPEERDMWTFMKENKILDMQQTYDMWLKKHNPGKPGEGTPISSREGEKQIQMPTDYADIMMGYHCWLCGKNSNSKKQWQQHIQSEKHKEKVFTSDSDASGWAFRFPMGEFRLCDRLQKGKACPDGDKCRCAHGQEE |
| 预测分子量 | 58.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于ZC3H7B重组蛋白的虚构参考文献(实际文献可能需要通过学术数据库检索)：
1. **文献名称**：ZC3H7B重组蛋白的纯化及其在RNA结合功能中的结构解析
**作者**：Kim S, et al.
**摘要**：本研究通过大肠杆菌表达系统纯化重组ZC3H7B蛋白，并利用X射线晶体学解析其CCCH锌指结构域的三维结构，揭示了其与特定RNA序列结合的分子机制。
2. **文献名称**：ZC3H7B与TTP蛋白互作在炎症反应中的调控作用
**作者**：Müller P, et al.
**摘要**：通过重组ZC3H7B蛋白的体外Pull-down实验，证实其与Tristetraprolin(TTP)的相互作用，协同调控促炎因子mRNA的稳定性，影响巨噬细胞的免疫应答。
3. **文献名称**：ZC3H7B重组蛋白在肝癌细胞增殖中的功能研究
**作者**：Chen L, et al.
**摘要**：利用重组ZC3H7B蛋白处理肝癌细胞系，发现其通过抑制PI3K/AKT信号通路降低细胞增殖能力，提示其作为肿瘤治疗潜在靶点的可能性。
(注：以上内容为模拟生成，实际文献需以具体数据库检索结果为准。)
ZC3H7B, also known as Zinc Finger CCCH-Type Containing 7B, is a protein encoded by the ZC3H7B gene in humans. It belongs to the CCCH-type zinc finger protein family, characterized by conserved cysteine and histidine residues that form structural motifs for nucleic acid binding, particularly RNA. ZC3H7B is implicated in post-transcriptional gene regulation, including mRNA stability, splicing, and turnover, often interacting with RNA-binding proteins or directly with RNA molecules to modulate cellular processes such as immune responses, cell cycle progression, and stress signaling. Studies suggest its involvement in pathways like NF-κB signaling, potentially linking it to inflammation and cancer progression.
Recombinant ZC3H7B protein refers to the engineered version produced in heterologous systems (e.g., E. coli, mammalian cells) for functional studies. This protein is typically purified using affinity tags (e.g., His-tag) and retains biological activity for in vitro assays. Researchers utilize recombinant ZC3H7B to investigate its RNA-binding specificity, structural features, and interactions with partner proteins or nucleic acids. It also serves as an antigen for antibody development or a tool to explore its role in diseases, including autoimmune disorders and malignancies. Recent work highlights its potential as a biomarker or therapeutic target, though detailed mechanisms remain under active investigation. Its conserved domains and regulatory functions make it a subject of interest in both basic and applied biomedical research.
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