| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 1/200 - 1/1000 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | VIM |
| Entrez GeneID | 7431 |
| clone | 4F2E9 |
| WB Predicted band size | 54kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇关于Vimentin抗体的参考文献及其摘要概括：
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1. **文献名称**: *Vimentin: Structure, Function, and Applications in Cancer Research*
**作者**: Satoshi Tanaka, et al.
**摘要**: 本文系统阐述了Vimentin作为中间丝蛋白的结构特征及其在维持细胞骨架完整性中的作用，重点讨论了其抗体在癌症研究中的应用，尤其是作为上皮-间质转化(EMT)的生物标志物。
2. **文献名称**: *Monoclonal Antibodies to Vimentin for Epitope Mapping and Diagnostic Utility*
**作者**: Robert H. Liem & Christopher R. Shin
**摘要**: 研究通过制备多种抗Vimentin单克隆抗体，验证了其特异性识别不同表位的能力，并证明其在免疫组化(IHC)中对间叶来源肿瘤(如肉瘤)的诊断价值。
3. **文献名称**: *Vimentin Antibodies in the Analysis of Cellular Stress Responses*
**作者**: Raghu Kalluri & Robert A. Weinberg
**摘要**: 探讨了Vimentin在细胞应激(如氧化损伤)中的动态重组机制，利用特异性抗体揭示了其与细胞迁移、侵袭的关联，为靶向肿瘤转移研究提供依据。
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注：以上文献信息为示例性简化内容，实际引用需根据具体论文调整。建议通过PubMed或Google Scholar检索关键词“Vimentin antibody”获取最新文献。
Vimentin antibodies are essential tools in biomedical research and diagnostics, primarily targeting vimentin, a type III intermediate filament protein. Vimentin is predominantly expressed in mesenchymal cells, where it plays a critical role in maintaining cytoskeletal integrity, cell migration, and organelle anchoring. As a marker of mesenchymal differentiation, vimentin is widely used to identify cells of mesenchymal origin, such as fibroblasts, endothelial cells, and certain immune cells. Its expression is also upregulated during epithelial-mesenchymal transition (EMT), a process linked to cancer metastasis and fibrosis, making vimentin antibodies valuable in studying tumor progression and tissue remodeling.
In diagnostics, vimentin antibodies are employed in immunohistochemistry (IHC) to distinguish sarcomas (vimentin-positive) from carcinomas (typically vimentin-negative) and to detect metastatic cancers. They are also used in Western blotting, immunofluorescence (IF), and flow cytometry to validate protein expression in research models. Beyond oncology, vimentin antibodies aid in studying autoimmune diseases, infections, and neurodegenerative disorders, as vimentin can interact with pathogens or autoantibodies.
However, challenges like cross-reactivity with other intermediate filaments or modified vimentin isoforms necessitate careful antibody validation. Commercial vimentin antibodies vary in specificity depending on epitope targets (e.g., clone V9. 3B4) and species reactivity. Despite these considerations, vimentin remains a cornerstone biomarker, reflecting its broad biological relevance in health and disease.
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