| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Histone H4 |
| Entrez GeneID | 121504 |
| WB Predicted band size | Calculated MW: 11 kDa; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S1 of human Histone H4.Email for sequence |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于CD45抗体的3篇代表性文献及其摘要的简要概述：
---
1. **文献名称**: "CD45: A critical regulator of signaling thresholds in immune cells"
**作者**: Hermiston ML, Xu Z, Weiss A
**摘要**: 该综述系统总结了CD45作为酪氨酸磷酸酶在免疫细胞信号调控中的核心作用，重点讨论了其对T细胞受体(TCR)和B细胞受体(BCR)信号通路的调节机制，以及CD45表达异常与免疫缺陷或自身免疫疾病的关联。
---
2. **文献名称**: "Monoclonal antibodies to human leucocyte antigens. II. Antibodies to CD45RA and CD45RO isoforms"
**作者**: Smith SH, Brown MH, Rowe D, et al.
**摘要**: 该研究报道了针对CD45不同亚型(如CD45RA和CD45RO)的单克隆抗体的开发与验证，通过流式细胞术和免疫印迹分析，揭示了这些抗体在区分T细胞亚群(如初始T细胞与记忆T细胞)中的特异性应用。
---
3. **文献名称**: "CD45 expression in acute leukemia: Diagnostic utility and correlation with immunophenotypic profiles"
**作者**: Dunphy CH, Tang W, Gonzalez M
**摘要**: 文章通过分析急性白血病患者的骨髓样本，评估CD45抗体在白血病分型中的临床价值，发现CD45表达水平差异可用于区分急性淋巴细胞白血病(ALL)和急性髓系白血病(AML)，并辅助微小残留病灶检测。
---
这些文献分别从分子机制、抗体开发及临床诊断角度探讨了CD45抗体的重要性。如需具体文章链接或扩展内容，可进一步通过PubMed或期刊数据库检索。
CD45. also known as leukocyte common antigen (LCA), is a transmembrane protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, excluding mature erythrocytes and platelets. It plays a critical role in regulating T-cell and B-cell receptor signaling by modulating the activity of Src-family kinases. Structurally, CD45 contains a variable extracellular domain generated by alternative splicing of exons 4-6 (resulting in isoforms like CD45RA, CD45RB, CD45RC, and CD45RO) and a conserved intracellular phosphatase domain. This molecular diversity correlates with functional differences in immune cell activation and differentiation.
CD45 antibodies are essential tools in immunology research and diagnostics. In flow cytometry, they serve as a pan-leukocyte marker to distinguish hematopoietic cells from non-hematopoietic populations. Differential CD45 expression patterns (e.g., bright in lymphocytes, dim in granulocytes) aid in immune cell subset identification. Clinically, CD45 antibodies are used in immunohistochemistry to characterize leukemias and lymphomas, as CD45 absence often indicates non-hematopoietic malignancies. Additionally, altered CD45 splicing or expression has been linked to autoimmune diseases, immunodeficiency, and viral infections (e.g., HIV), making it a biomarker of interest. Therapeutic CD45-targeting strategies, though experimental, are being explored for immunosuppression in transplantation.
×
