| WB | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat,Rabbit |
| ICC | 技术咨询 | Human,Mouse,Rat,Rabbit |
| FCM | 咨询技术 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Aliases | P16 |
| Entrez GeneID | 1029 |
| clone | 2D9A12 |
| WB Predicted band size | 16.5kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of P16 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠与人类P16抗体的3篇参考文献，涵盖抗体应用与机制研究：
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1. **标题**：Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers
**作者**：Nobori T, et al.
**摘要**：该研究通过免疫组化分析，发现P16(CDKN2A)基因缺失与多种人类癌症(如黑色素瘤、肺癌)相关。研究使用小鼠与人类细胞系验证抗体特异性，证实P16蛋白缺失促进肿瘤发生。
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2. **标题**：Overexpression of p16(INK4A) as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri
**作者**：Klaes R, et al.
**摘要**：研究验证了P16抗体在宫颈癌前病变和癌变组织中的高表达，对比小鼠模型与人类临床样本，表明P16过表达与HPV感染相关，可作为宫颈癌诊断的生物标志物。
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3. **标题**：Loss of p16Ink4a with retention of p19Arf predisposes mice to tumorigenesis
**作者**：Sharpless NE, et al.
**摘要**：通过基因工程小鼠模型，研究P16INK4a特异性缺失如何导致肿瘤易感性增加，同时验证了人类肿瘤中P16表达缺失的类似机制，强调了抗体在检测蛋白表达缺失中的关键作用。
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这些文献涵盖了P16抗体在癌症机制、诊断标志物及跨物种比较中的应用，提供理论与实验参考。
The p16 antibody, targeting both mouse and human p16 proteins (also known as CDKN2A or INK4a), is a critical tool in studying cell cycle regulation and tumor suppression. The p16 protein functions as a cyclin-dependent kinase (CDK) inhibitor, blocking CDK4/6 activity to prevent phosphorylation of the retinoblastoma (Rb) protein, thereby arresting the cell cycle at the G1 phase. Dysregulation of p16 is closely linked to cancer development, as its inactivation (via mutations, deletions, or promoter methylation) disrupts cell cycle control, promoting uncontrolled proliferation. Conversely, overexpression of p16 is observed in aging cells and premalignant lesions, serving as a biomarker of cellular senescence or oncogenic stress.
Anti-p16 antibodies are widely used in research and diagnostics to detect p16 expression in tissues or cell lines via techniques like immunohistochemistry (IHC), Western blotting, or immunofluorescence (IF). In human cancers, such as cervical carcinoma, p16 upregulation is strongly associated with high-risk HPV infections, making it a diagnostic marker for HPV-related neoplasia. In mouse models, p16 antibodies help study aging, senescence, and tumorigenesis.
These antibodies are typically raised against conserved epitopes shared between human and mouse p16. ensuring cross-reactivity. Validation includes specificity testing in knockout models and applications across multiple platforms. Their versatility supports both basic research into cell cycle mechanisms and clinical applications in cancer prognosis and therapeutic targeting.
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