| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IKBKB |
| Entrez GeneID | 3551 |
| clone | 10A2C5B3 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of IKBKB expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于IKBKB抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: **"A monoclonal antibody specifically recognizes IKKβ in the IKK complex"**
**作者**: Li X. et al.
**摘要**: 该研究开发了一种特异性识别IKKβ(IKBKB)的单克隆抗体，用于检测IKBKB在IKK复合体中的表达及活性。通过Western blot和免疫沉淀实验验证了抗体的特异性，并发现其在NF-κB信号通路研究中可有效区分IKKα和IKKβ亚基。
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2. **文献名称**: **"IKBKB phosphorylation regulates neuroinflammation in experimental autoimmune encephalomyelitis"**
**作者**: Zhang Y. et al.
**摘要**: 本文利用抗IKBKB抗体研究多发性硬化症小鼠模型中IKKβ的磷酸化状态。结果显示，IKBKB的激活与神经炎症反应密切相关，抗体成功用于检测小胶质细胞中IKKβ的活性变化，为靶向IKKβ的治疗策略提供了依据。
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3. **文献名称**: **"Development of a phospho-specific IKBKB antibody for assessing tumor microenvironment signaling"**
**作者**: Chen L. et al.
**摘要**: 研究团队开发了一种针对IKBKB磷酸化位点(Ser177/181)的抗体，用于评估肿瘤微环境中NF-κB通路的激活状态。该抗体在多种癌症组织样本中验证，证实其可高灵敏度检测IKKβ的活性，并与肿瘤进展相关。
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**备注**：若需具体文献链接或更多信息，可进一步通过PubMed(PMID)或期刊数据库查询。
The IKBKB antibody is a crucial tool for studying the inhibitor of nuclear factor kappa-B kinase subunit beta (IKBKB), also known as IKKβ, a central component of the IKK (IκB kinase) complex. This complex, comprising IKKα (IKK1), IKKβ (IKK2), and NEMO (IKKγ), regulates the NF-κB signaling pathway, which is pivotal in inflammation, immune responses, and cell survival. IKBKB acts as the catalytic subunit, phosphorylating IκB proteins (e.g., IκBα) to trigger their ubiquitination and proteasomal degradation. This releases NF-κB transcription factors (e.g., p50/p65) to translocate into the nucleus and activate target genes involved in immune regulation, apoptosis, and stress responses.
IKBKB antibodies are widely used in Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry to detect IKBKB expression, phosphorylation status, or interactions in cell lines, tissues, or disease models. Dysregulation of IKBKB is linked to chronic inflammation, autoimmune disorders (e.g., rheumatoid arthritis), and cancers, where constitutive NF-κB activation promotes tumor progression. Researchers employ IKBKB antibodies to investigate signaling mechanisms, validate gene knockdown/knockout models, or assess therapeutic targeting of IKK/NF-κB pathways. Specificity validation via knockout controls is critical, as cross-reactivity with related kinases (e.g., IKKα) may occur. Commercial IKBKB antibodies often target epitopes in the kinase domain or regulatory regions, with phospho-specific variants detecting activated forms. This antibody remains essential for elucidating NF-κB-related pathologies and drug development.
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