| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYNC1I1 |
| Uniprot No | O14576 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-645aa |
| 氨基酸序列 | MSDKSDLKAELERKKQRLAQIREEKKRKEEERKKKEADMQQKKEPVQDDSDLDRKRRETEALLQSIGISPEPPLVQPLHFLTWDTCYFHYLVPTPMSPSSKSVSTPSEAGSQDSGDLGPLTRTLQWDTDPSVLQLQSDSELGRRLHKLGVSKVTQVDFLPREVVSYSKETQTPLATHQSEEDEEDEEMVESKVGQDSELENQDKKQEVKEAPPRELTEEEKQQILHSEEFLIFFDRTIRVIERALAEDSDIFFDYSGRELEEKDGDVQAGANLSFNRQFYDEHWSKHRVVTCMDWSLQYPELMVASYNNNEDAPHEPDGVALVWNMKFKKTTPEYVFHCQSSVMSVCFARFHPNLVVGGTYSGQIVLWDNRSHRRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLITVSTDGKMCSWSLDMLSTPQESMELVYNKSKPVAVTGMAFPTGDVNNFVVGSEEGTVYTACRHGSKAGIGEVFEGHQGPVTGINCHMAVGPIDFSHLFVTSSFDWTVKLWTTKHNKPLYSFEDNADYVYDVMWSPVHPALFACVDGMGRLDLWNLNNDTEVPTASVAIEGASALNRVRWAQAGKEVAVGDSEGRIWVYDVGELAVPHNDEWTRFARTLVEIRANRADSEEEGTVELSA |
| 预测分子量 | 75.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DYNC1I1重组蛋白的3篇模拟参考文献(基于领域知识概括，非真实文献)：
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1. **文献名称**：*Structural Insights into DYNC1I1 Intermediate Chain Function in Cytoplasmic Dynein Complex Assembly*
**作者**：Smith J. et al.
**摘要**：本研究通过重组表达DYNC1I1蛋白，结合X射线晶体学解析其结构，揭示了其与动力蛋白重链的结合界面，阐明了其在驱动细胞内物质运输复合体组装中的关键作用。
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2. **文献名称**：*DYNC1I1 Knockdown Disrupts Mitotic Spindle Organization in Human Cancer Cells*
**作者**：Li X. et al.
**摘要**：利用重组DYNC1I1蛋白进行体外功能补偿实验，发现其缺失导致有丝分裂纺锤体异常，表明其在维持染色体分离和癌细胞增殖中的必要性。
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3. **文献名称**：*Recombinant DYNC1I1 Facilitates Neuronal Vesicle Transport in Axon Regeneration Models*
**作者**：Chen R. et al.
**摘要**：通过体外表达DYNC1I1重组蛋白并导入神经元模型，证实其可增强轴突囊泡运输效率，为神经退行性疾病治疗提供了潜在靶点。
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**注意**：以上为模拟文献摘要，实际研究需通过学术数据库(如PubMed、Google Scholar)检索真实文献。
DYNC1I1 recombinant protein is derived from the cytoplasmic dynein 1 intermediate chain 1. a critical component of the dynein motor complex. Cytoplasmic dynein is a multisubunit molecular motor responsible for retrograde intracellular transport along microtubules, facilitating processes such as organelle positioning, mitotic spindle organization, and cargo trafficking. The DYNC1I1 gene encodes an intermediate chain that plays a structural and regulatory role in the dynein complex, mediating interactions between the heavy chain and light chains or cargo adaptors. Dysfunctions in dynein components, including DYNC1I1. have been implicated in neurodevelopmental disorders, neurodegenerative diseases, and cancer metastasis.
Recombinant DYNC1I1 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling controlled study of its biochemical properties. Purification methods often involve affinity tags (e.g., GST, His-tag) followed by chromatography. This engineered protein serves as a tool to investigate dynein assembly mechanisms, cargo-binding specificity, and regulatory post-translational modifications. Researchers utilize it in *in vitro* binding assays, structural studies (e.g., cryo-EM), and functional reconstitution experiments to dissect dynein-mediated transport pathways. It also aids in identifying interacting partners through pull-down assays or yeast two-hybrid screens. In disease research, DYNC1I1 recombinant protein helps model mutations linked to cortical malformations or ciliopathies, providing insights into pathological mechanisms. Additionally, it supports therapeutic development by serving as a target for drug screening or antibody production aimed at modulating dynein activity in cancer or neurological conditions.
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