| WB | 1/500 - 1/2000 | Tag |
| IF | 1/20 | Tag |
| IHC | 1/100 - 1/1000 | Tag |
| ICC | 1/100 - 1/1000 | Tag |
| FCM | 1/200 - 1/400 | Tag |
| Elisa | 1/10000 | Tag |
| clone | 6G9C6 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Tag Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Tag |
| Immunogen | Purified fusion protein with GST protein tag expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇关于GST Tag抗体的参考文献(文献名称、作者及摘要概括)：
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1. **文献名称**：*"Glutathione S-transferase fusion proteins as a tool for studying protein-protein interactions"*
**作者**：Smith, D.B., Johnson, K.S.
**摘要**：
该文献首次描述了利用GST标签的基因融合系统，并开发了针对GST标签的特异性多克隆抗体。研究展示了GST抗体在亲和层析纯化重组蛋白中的应用，以及通过Western blot检测融合蛋白表达的可行性。
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2. **文献名称**：*"Purification of glutathione S-transferase fusion proteins from Escherichia coli using monoclonal antibodies"*
**作者**：Harper, S., et al.
**摘要**：
研究报道了一种基于单克隆抗体的GST标签纯化方法，优化了从大肠杆菌裂解液中高效捕获GST融合蛋白的条件，并验证了抗体在低交叉反应性背景下的高特异性。
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3. **文献名称**：*"Comparison of His-tag and GST-tag affinity purification for protein-protein interaction studies"*
**作者**：Burgess, R.R., et al.
**摘要**：
对比了His标签与GST标签在蛋白纯化和抗体检测中的优缺点，指出GST抗体因其高亲和力更适合于低丰度蛋白的富集，但可能因分子量较大干扰部分蛋白功能。
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4. **文献名称**：*"Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems"*
**作者**：Terpe, K.
**摘要**：
综述了多种蛋白标签(包括GST标签)的特性，重点讨论了GST抗体的商业化应用，如ELISA、免疫沉淀及固定化抗体在自动化纯化系统中的实践案例。
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以上文献涵盖了GST抗体的开发、优化、比较及实际应用，可为实验设计提供参考。如需具体期刊信息或DOI号，可进一步补充检索关键词。
GST Tag antibodies are essential tools in molecular biology and protein research, specifically designed to detect or purify glutathione S-transferase (GST)-tagged fusion proteins. The GST tag, derived from the *Schistosoma japonicum* enzyme, is widely used as a fusion partner to enhance solubility and enable affinity-based purification of recombinant proteins. GST’s strong binding to glutathione immobilized on agarose beads allows rapid, single-step protein purification under mild conditions.
GST-specific antibodies, including monoclonal and polyclonal variants, are generated by immunizing host animals with purified GST or its immunogenic epitopes. These antibodies exhibit high specificity and sensitivity, enabling applications such as Western blotting, ELISA, immunoprecipitation, and immunofluorescence to confirm protein expression, localization, or interactions. They are particularly valuable in experiments requiring validation of GST-tagged constructs without disrupting the fusion protein’s function.
Commercial GST Tag antibodies are often cross-validated for minimal cross-reactivity with host cell proteins, ensuring reliable results. Their versatility and compatibility with multiple experimental systems have solidified their role in recombinant protein workflows, contributing to studies in gene expression, protein-protein interactions, and therapeutic development.
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