| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| clone | 3G3A2 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Immunogen | Bovine serum albumin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BSA抗体的3篇示例参考文献(内容为虚构，供参考)：
1. **文献名称**：*Cross-reactivity of Anti-BSA Antibodies in Immunoassays: Challenges and Solutions*
**作者**：Smith, J., et al.
**摘要**：该研究探讨了BSA作为载体蛋白在免疫实验中引发的抗体交叉反应问题，分析了不同实验条件下抗BSA抗体的干扰机制，并提出通过优化封闭剂或使用替代蛋白来减少假阳性结果。
2. **文献名称**：*Production and Characterization of Polyclonal Antibodies Against Bovine Serum Albumin*
**作者**：Lee, H., & Zhang, Y.
**摘要**：文章描述了一种高效制备兔源多克隆抗BSA抗体的方法，通过免疫原设计、效价检测及特异性验证，证明该抗体在Western blot和ELISA中具有高灵敏度和低非特异性结合的特点。
3. **文献名称**：*BSA Interference in Diagnostic Assays: A Meta-Analysis*
**作者**：Brown, T., et al.
**摘要**：通过对现有文献的荟萃分析，总结BSA在临床诊断试剂(如ELISA)中可能引起的抗体干扰问题，强调在试剂开发中需严格评估BSA批次差异对检测结果的影响。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词如**"BSA antibody cross-reactivity"**或**"anti-BSA antibody applications"**获取。
Bovine serum albumin (BSA) antibodies are immunoglobulins specifically designed to recognize and bind to BSA, a highly abundant protein derived from bovine blood plasma. BSA, with a molecular weight of approximately 66.5 kDa, is widely utilized in laboratory settings as a carrier protein, blocking agent, or stabilizer in immunoassays (e.g., ELISA, Western blot) and diagnostic kits. Its structure comprises three homologous domains rich in alpha-helices and 17 disulfide bonds, contributing to stability and solubility.
BSA antibodies are typically generated by immunizing animals (e.g., rabbits, mice, goats) with purified BSA, followed by polyclonal or monoclonal antibody production. Polyclonal antibodies target multiple epitopes, enhancing detection sensitivity, while monoclonal antibodies offer specificity for a single epitope. These antibodies are critical for detecting BSA contamination in biopharmaceuticals, vaccines, or cell culture media, where residual BSA from production processes must be quantified for quality control.
However, cross-reactivity with homologous proteins like human serum albumin (HSA) can occur due to structural similarities, necessitating validation for assay specificity. BSA antibodies also serve as controls in immunostaining or as calibration tools in quantitative assays. Proper storage at -20°C and avoidance of repeated freeze-thaw cycles preserve antibody functionality. Their versatility and broad applicability make BSA antibodies essential reagents in both research and industrial bioprocessing.
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