| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NR4A2 |
| Uniprot No | P43354 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-598aa |
| 氨基酸序列 | MPCVQAQYGSSPQGASPASQSYSYHSSGEYSSDFLTPEFVKFSMDLTNTEITATTSLPSFSTFMDNYSTGYDVKPPCLYQMPLSGQQSSIKVEDIQMHNYQQHSHLPPQSEEMMPHSGSVYYKPSSPPTPTTPGFQVQHSPMWDDPGSLHNFHQNYVATTHMIEQRKTPVSRLSLFSFKQSPPGTPVSSCQMRFDGPLHVPMNPEPAGSHHVVDGQTFAVPNPIRKPASMGFPGLQIGHASQLLDTQVPSPPSRGSPSNEGLCAVCGDNAACQHYGVRTCEGCKGFFKRTVQKNAKYVCLANKNCPVDKRRRNRCQYCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKSPQEPSPPSPPVSLISALVRAHVDSNPAMTSLDYSRFQANPDYQMSGDDTQHIQQFYDLLTGSMEIIRGWAEKIPGFADLPKADQDLLFESAFLELFVLRLAYRSNPVEGKLIFCNGVVLHRLQCVRGFGEWIDSIVEFSSNLQNMNIDISAFSCIAALAMVTERHGLKEPKRVEELQNKIVNCLKDHVTFNNGGLNRPNYLSKLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPAIIDKLFLDTLPF |
| 预测分子量 | 68.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NR4A2重组蛋白的参考文献摘要示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Recombinant NR4A2 Protein Enhances Dopaminergic Neuron Survival in Parkinson's Disease Models"*
**作者**: Smith J, et al. (2020)
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化NR4A2重组蛋白，证实其可激活多巴胺能神经元特异性基因转录。体外实验表明，重组NR4A2显著抑制神经毒素诱导的神经元凋亡，提示其潜在神经保护作用。
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2. **文献名称**: *"Structural and Functional Characterization of the NR4A2 Ligand-Binding Domain"*
**作者**: Lee H, et al. (2018)
**摘要**: 利用昆虫细胞系统表达NR4A2配体结合域(LBD)重组蛋白，通过X射线晶体学解析其三维结构。功能实验表明，该结构域与特定配体结合后可调控靶基因表达，为开发NR4A2靶向药物提供结构基础。
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3. **文献名称**: *"NR4A2 Recombinant Protein Attenuates Inflammatory Responses in Microglial Cells"*
**作者**: Zhang R, et al. (2021)
**摘要**: 通过哺乳动物细胞系制备高纯度NR4A2重组蛋白，发现其可抑制脂多糖激活的小胶质细胞中NF-κB通路活性，减少促炎因子释放。机制研究提示NR4A2通过直接结合炎症相关基因启动子发挥抗炎作用。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“NR4A2 recombinant protein”检索最新研究。
NR4A2 (Nuclear Receptor Subfamily 4 Group A Member 2), also known as Nurr1. is a transcription factor belonging to the nuclear receptor superfamily. Unlike most nuclear receptors, NR4A2 lacks a classical ligand-binding domain, suggesting its activation may occur through ligand-independent mechanisms or via atypical signaling pathways. It plays critical roles in regulating gene expression by binding to specific DNA sequences, particularly in the nervous and immune systems. NR4A2 is essential for the development, maintenance, and function of dopaminergic neurons in the midbrain, making it a key player in Parkinson’s disease pathogenesis. Dysregulation of NR4A2 has also been implicated in neuroinflammation, cancer progression, and metabolic disorders.
Recombinant NR4A2 protein is engineered using molecular cloning techniques, typically expressed in bacterial, insect, or mammalian systems to ensure proper folding and post-translational modifications. This purified protein retains functional domains, including the DNA-binding domain (DBD) and the N-terminal activation domain, enabling its use in biochemical assays, structural studies, and interaction analyses. Researchers employ recombinant NR4A2 to investigate its transcriptional regulatory mechanisms, screen for potential modulators, or study its role in cellular pathways. In drug discovery, it serves as a tool to develop therapies targeting NR4A2-associated diseases, such as neurodegenerative disorders or inflammatory conditions. Its production often involves affinity tags (e.g., GST, His-tag) for simplified purification and detection. By providing a controlled and scalable source of the protein, recombinant NR4A2 accelerates both basic research and translational applications, bridging gaps between molecular understanding and therapeutic innovation.
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