| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPFFR2 |
| Uniprot No | A0PJM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420kDa |
| 氨基酸序列 | MNEKWDTNSSENWHPIWNVNDTKHHLYSDINITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQNKTSPVYWCREDWPNQEMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLFRAAVPHTGRKNQEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYADLSPNELQIINIYIYPFAHWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSHVLINTSNQLVQESTFQNPHGETLLYRKSAEKPQQELVMEELKETTNSSEI |
| 预测分子量 | 48,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPFFR2重组蛋白的3篇参考文献示例(注：文献信息为示例性概括，具体内容需根据实际文献调整)：
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1. **文献名称**：**"Recombinant expression and functional characterization of human NPFFR2 in HEK293 cells"**
**作者**：Wang, Y., et al.
**摘要**：本研究在HEK293细胞中成功表达并纯化了人源NPFFR2重组蛋白，通过钙流实验证实其与神经肽FF(NPFF)的特异性结合及下游G蛋白信号通路的激活，为受体药理学研究提供工具。
2. **文献名称**：**"Structural basis of NPFFR2 activation by neuropeptide FF revealed by cryo-EM"**
**作者**：Zhang, L., et al.
**摘要**：利用冷冻电镜技术解析了NPFFR2与其天然配体NPFF结合的复合物结构，揭示了受体激活的构象变化机制，为靶向NPFFR2的药物设计提供结构基础。
3. **文献名称**：**"NPFFR2 modulates opioid tolerance: Insights from recombinant receptor studies in vitro"**
**作者**：Gauvreau, R., et al.
**摘要**：通过体外重组NPFFR2模型，证明其在阿片类药物耐受中的作用，发现NPFFR2与μ-阿片受体存在交叉调控，提示其作为镇痛辅助靶点的潜力。
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如需获取具体文献，建议通过PubMed、Google Scholar等平台检索关键词“NPFFR2 recombinant”、“NPFFR2 expression”或结合研究领域(如疼痛、GPCR信号)。早期研究(如2000年代初)可能涵盖受体克隆与基础功能分析。
**Background of NPFFR2 Recombinant Protein**
NPFFR2 (Neuropeptide FF Receptor 2), also known as GPR74 or HLWAR77. is a G protein-coupled receptor (GPCR) that primarily binds neuropeptide FF (NPFF) and related peptides. It plays a role in modulating pain perception, opioid regulation, stress responses, and energy homeostasis. NPFFR2 is expressed in the central nervous system, particularly in regions like the hypothalamus, spinal cord, and amygdala, linking it to neuroendocrine functions and behavioral adaptations.
Structurally, NPFFR2 contains seven transmembrane domains and signals through Gi/o proteins, inhibiting cAMP production upon ligand activation. Its interactions with NPFF and RFamide-related peptides (RFRPs) are critical for regulating neurotransmitter release and synaptic plasticity. Dysregulation of NPFFR2 has been implicated in chronic pain syndromes, addiction, and metabolic disorders, making it a potential therapeutic target.
Recombinant NPFFR2 protein is produced in vitro using expression systems like mammalian or insect cells to ensure proper post-translational modifications. It is often tagged (e.g., His-tag, FLAG) for purification and detection. This engineered protein retains native receptor conformation and ligand-binding activity, enabling functional studies such as ligand-receptor interaction assays, signal transduction analysis, and high-throughput drug screening. Researchers also utilize it to explore structural dynamics via crystallography or cryo-EM, aiding in the design of receptor-specific agonists or antagonists.
Overall, NPFFR2 recombinant protein serves as a vital tool for deciphering the receptor’s physiological roles and developing treatments for pain, addiction, and metabolic diseases. Its application bridges molecular biology and translational research, offering insights into GPCR-mediated pathways.
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