| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PTP3 |
| Uniprot No | P54637 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-990aa |
| 氨基酸序列 | MISSSMSYRHSTNSVYTLNPHLNIPISTSTTIPPTSFYANNTPEMIQSQSENTNTNNINNSSSNINNNNNNTPDSMSMSTSLSSSPSVSFNHLDLNSINNKINNNTTTNNNNNNNNNNDDKFDTNALKLSNTMIIKNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNSNIEINVPSIQFDNEPAMEVDSVAPLNVPSNHTRTTLAMHNTKSLSTSNIGLLNILPNQQSSSSSSLSSTTTTTTTTSSSLLMPQSLFNNSTYNNHHNNNNSSNAGIVGGLNGSTSSLPTQAQVQLQQMQQQMQQHQQHQYKKANLSSLSTVVDNNLNNNPMNTSTSSPAQPNASPFSFSSSSLFSNSSLSNSGSGSASTTSTSTSSSNSMSSSPPPSLKTSFSQLDEDREKMRLEFEMIKKPEMASKKSHKHHQRHYSHNDLDNRKHDEEKFFSALQPNNYGKNRYHDVLPNESTRVRLTPIESGDGDYINANYINGEVPNSYRYYIACQAPLPSTIKDFWRMVWEERSSVIVCLTKLEENGKKKADVYYPETSQAQEYGSFWIHLHKKVMFKDIGVSSLHLYKKGEEFPREVVLLHYTQWPDCGAPPSSSHIRTLSVMVNTFKARGSAKNTNGPVIVHCSAGIGRSGTFISININMAKIERFGNDPSQMNISIKDSVLELRRQRRGMVQTLDQYIFIFKVINDVLTDMGIRSLSSPSKRRSCEMIKSTPMPRLDISIPPPLTFTPKDFQSSISPSTDMIASLSIITQMTQTLKFPPQQQQDNPFSKSSIKISPSPLNSTNISIPKNQQFQHPFQIQPQLDLNLQQQQQQSSQQLNDNPPLNMSSNSIKFPPVTSLSSCHLFEDSKNNDNNNKQQQQQQQQQQKNNQQCSGFSHFLNNNNNNDNNGSSGGGFNGSFLFNSNNSGSSSTNSECSNNNKNNNNNSNNNNNNNNNKNSDNNGTKDKDENDSCESPRVTPIKCF |
| 预测分子量 | 110 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PTP3(Protein Tyrosine Phosphatase 3)重组蛋白相关的参考文献及其简要摘要：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant PTP3 in Drosophila Development"*
**作者**：Hoffmann, A., et al.
**摘要**：本研究通过昆虫细胞表达系统成功制备了重组Drosophila PTP3蛋白，并验证其在胚胎发育中对EGFR信号通路的负调控作用，揭示了PTP3通过去磷酸化关键受体调控细胞分化。
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2. **文献名称**：*"Structural Insights into Human PTP3 (PTPRG) Reveal a Novel Regulatory Mechanism in Cancer Metastasis"*
**作者**：Zhang, Y., et al.
**摘要**：通过大肠杆菌表达纯化人源PTP3重组蛋白，结合晶体结构分析和体外酶活实验，发现其胞外域与细胞粘附分子互作，可能通过抑制Wnt/β-catenin通路抑制肿瘤转移。
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3. **文献名称**：*"Recombinant PTP3 as a Therapeutic Target in Autoimmune Diseases: In Vitro and In Vivo Validation"*
**作者**：Lee, S., & Kim, J.
**摘要**：利用哺乳动物HEK293系统表达功能性PTP3重组蛋白，发现其通过调控T细胞受体(TCR)信号通路抑制过度免疫反应，为类风湿性关节炎治疗提供潜在靶点。
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4. **文献名称**：*"High-Yield Production of Active PTP3 in Yeast: Applications in Drug Screening"*
**作者**：García-Ruiz, E., et al.
**摘要**：通过毕赤酵母系统高效表达具有活性的重组PTP3蛋白，并建立基于该蛋白的抑制剂筛选平台，鉴定出多个小分子化合物可特异性增强其磷酸酶活性。
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注：以上文献为示例性内容，实际文献需根据具体研究领域(如物种、疾病模型等)进一步检索确认。
**Background of PTP3 Recombinant Protein**
Protein tyrosine phosphatase 3 (PTP3), a member of the protein tyrosine phosphatase (PTP) superfamily, plays a critical role in regulating cellular signaling by dephosphorylating tyrosine residues on target proteins. PTPs counterbalance the activity of tyrosine kinases, maintaining the equilibrium of phosphorylation states essential for processes like cell growth, differentiation, and apoptosis. PTP3. also referred to as PTPRG or PTPγ in certain contexts, is characterized by a conserved catalytic domain and extracellular receptor-like regions, suggesting potential roles in cell-cell communication and ligand binding.
Recombinant PTP3 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional enzyme for research. Its recombinant form enables precise study of substrate specificity, enzymatic kinetics, and interactions with signaling pathways, particularly in cancer and neurological disorders. Dysregulation of PTP3 has been linked to tumor suppression, immune modulation, and synaptic plasticity, making it a focus for therapeutic targeting.
Studies highlight PTP3's involvement in dephosphorylating receptor tyrosine kinases (RTKs) and adhesion molecules, influencing cell migration and metastasis. Its recombinant variants, including catalytically inactive mutants, serve as tools to dissect phosphorylation-dependent mechanisms in vitro or in vivo. Research on PTP3 also explores its regulatory crosstalk with oncogenic pathways like EGFR and MET, underscoring its dual role as a tumor suppressor or promoter in context-dependent scenarios.
Overall, recombinant PTP3 remains pivotal in deciphering tyrosine phosphorylation networks and developing strategies to modulate PTP activity in disease.
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