| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCR10 |
| Uniprot No | Q9UDW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-63aa |
| 氨基酸序列 | AAATLTSKLYSLLFRRTSTFALTIIVGVMFFERAFDQGADAIYDHINEGKLWKHIKHKYENK |
| 预测分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UQCR10重组蛋白的3条参考文献示例(内容基于学术领域常见研究方向，具体文献需通过数据库核实)：
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1. **标题**: *"Recombinant expression and functional characterization of UQCR10 in mitochondrial complex III assembly"*
**作者**: Smith A, et al.
**摘要**: 研究通过大肠杆菌系统成功表达重组UQCR10蛋白，并证明其在线粒体复合体III组装中的关键作用，缺乏UQCR10导致电子传递链活性下降。
2. **标题**: *"Structural insights into the role of UQCR10 in cytochrome bc1 complex by cryo-EM"*
**作者**: Zhang Y, et al.
**摘要**: 利用冷冻电镜解析包含重组UQCR10的人源细胞色素bc1复合体(复合体III)结构，揭示UQCR10在稳定复合体构象及质子传递中的分子机制。
3. **标题**: *"UQCR10 overexpression promotes tumorigenesis by modulating mitochondrial ROS in colorectal cancer"*
**作者**: Lee H, et al.
**摘要**: 通过重组UQCR10蛋白实验，发现其过表达增强线粒体活性氧(ROS)生成，促进结直肠癌细胞增殖，提示其作为潜在治疗靶点。
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**注意**：以上文献为示例，实际研究需通过PubMed、Google Scholar等平台以“UQCR10 recombinant protein”、“UQCR10 complex III”等关键词检索确认。
UQCR10 (Ubiquinol-Cytochrome c Reductase Complex Subunit 10) is a nuclear-encoded mitochondrial protein that plays a critical role in the electron transport chain (ETC), specifically as a component of Complex III (cytochrome bc1 complex). This complex is essential for oxidative phosphorylation, facilitating electron transfer from ubiquinol to cytochrome c while contributing to the proton gradient across the mitochondrial inner membrane, which drives ATP synthesis. UQCR10 is one of the 11 subunits of Complex III, though its exact functional role remains less characterized compared to core subunits like cytochrome b or the Rieske protein. Studies suggest it may assist in stabilizing the complex’s structure or modulating its activity.
Recombinant UQCR10 protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cell lines) to enable functional and structural studies. Its production allows researchers to investigate UQCR10’s interactions within Complex III, its role in mitochondrial bioenergetics, and potential links to diseases. Dysregulation of UQCR10 has been implicated in conditions such as cancer, neurodegenerative disorders, and metabolic syndromes. For instance, reduced UQCR10 expression correlates with impaired mitochondrial respiration in certain cancers, while mutations may disrupt ETC efficiency, leading to oxidative stress.
The recombinant protein is also utilized to develop antibodies, study protein-protein interactions, or screen therapeutic compounds targeting mitochondrial dysfunction. Recent advances in cryo-EM and structural biology have heightened interest in UQCR10’s 3D configuration to elucidate mechanistic details of Complex III assembly and function. Despite its peripheral characterization, UQCR10 represents a vital piece of the mitochondrial energy production puzzle, with ongoing research aimed at uncovering its precise contributions to cellular health and disease.
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