Recombinant Human KRT35 protein

中文名： 角蛋白，I型角质层Ha5(KRT35)重组蛋白
别名： KRT35；HHA5；HKA5；KRTHA5；Keratin, type I cuticular Ha5

货号: PA2000-3348

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	KRT35
Uniprot No	Q92764
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-455aa
氨基酸序列	MASKCLKAGFSSGSLKSPGGASGGSTRVSAMYSSSSCKLPSLSPVARSFSACSVGLGRSSYRATSCLPALCLPAGGFATSYSGGGGWFGEGILTGNEKETMQSLNDRLAGYLEKVRQLEQENASLESRIREWCEQQVPYMCPDYQSYFRTIEELQKKTLCSKAENARLVVEIDNAKLAADDFRTKYETEVSLRQLVESDINGLRRILDDLTLCKSDLEAQVESLKEELLCLKKNHEEEVNSLRCQLGDRLNVEVDAAPPVDLNRVLEEMRCQYETLVENNRRDAEDWLDTQSEELNQQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHSMRDALESTLAETEARYSSQLAQMQCMITNVEAQLAEIRADLERQNQEYQVLLDVRARLECEINTYRGLLESEDSKLPCNPCAPDYSPSKSCLPCLPAASCGPSAARTNCSPRPICVPCPGGRF
预测分子量	66.4 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是模拟生成的3篇关于KRT35重组蛋白的参考文献示例(非真实文献，仅供格式参考)：

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1. **文献名称**: *Cloning and Expression of Recombinant Human KRT35 in Escherichia coli*

**作者**: Smith A, et al.

**摘要**: 本研究成功克隆了人类KRT35基因，并利用大肠杆菌表达系统制备了重组KRT35蛋白。通过His标签纯化和质谱验证，获得了高纯度蛋白，为后续研究其与毛囊角蛋白相关疾病的相互作用提供了工具。

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2. **文献名称**: *Structural Analysis of KRT35 Reveals Its Role in Hair Shaft Integrity*

**作者**: Lee H, et al.

**摘要**: 通过重组KRT35蛋白的晶体结构解析，发现其α-螺旋结构域对维持毛发角蛋白纤维的稳定性至关重要。体外实验表明，KRT35突变体会导致角蛋白网络组装异常，提示其在遗传性毛发疾病中的潜在机制。

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3. **文献名称**: *KRT35 Mutations and Their Impact on Keratin Filament Formation*

**作者**: Patel R, et al.

**摘要**: 本研究利用重组KRT35蛋白模拟了先天性厚甲症相关突变体(如p.Leu132Pro)，发现突变蛋白在体外形成异常纤维结构，并通过细胞实验证实其干扰角蛋白细胞骨架网络，为疾病机理提供了分子证据。

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**备注**：以上文献为模拟内容，如需真实文献建议通过以下途径查询：

1. **PubMed**：https://pubmed.ncbi.nlm.nih.gov

2. **Google Scholar**：https://scholar.google.com

搜索关键词：*KRT35 recombinant protein, KRT35 expression, Keratin 35 gene cloning*

背景信息

Keratins are a family of structural proteins that form intermediate filaments, crucial for maintaining cellular integrity and mechanical stability in epithelial cells and hair-related tissues. KRT35. encoding keratin 35. is a type I acidic keratin specifically expressed in the hair cortex and nail matrix. It pairs with type II keratins (e.g., KRT85) to assemble into heteropolymers, contributing to the resilience of hair shafts and nail plates. Mutations in KRT35 are linked to hereditary hair/nail disorders, such as monilethrix (beaded hair) and congenital nail dysplasia, highlighting its role in keratinocyte differentiation and cytoskeletal organization.

Recombinant KRT35 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to produce purified, functional KRT35 for research and therapeutic development. Its production typically involves cloning the human KRT35 gene into expression vectors, followed by protein extraction and purification via affinity chromatography (e.g., His-tag systems). Challenges include ensuring proper folding and post-translational modifications, which may require eukaryotic systems.

Studies leveraging recombinant KRT35 focus on elucidating its structural interactions with binding partners, disease mechanisms caused by mutations, and screening compounds to restore keratin network integrity. It also aids in generating antibodies for diagnostic assays and exploring gene therapy strategies for keratinopathies. As a research tool, recombinant KRT35 bridges molecular insights with clinical applications in dermatology and genetic medicine.