| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABHD18 |
| Uniprot No | Q0P651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-414aa |
| 氨基酸序列 | RPEDLK RLFEFRKMIG NRERCQNLVS SDYPVHIDKI EEQSDCKILD GHFVSPMAHY VPDIMPIESV IARFQFIVPK EWNSKYRPVC IHLAGTGDHH YWRRRTLMAR PMIKEARMAS LLLENPYYGC RKPKDQVRSS LKNVSDLFVM GGALVLESAA LLHWLEREGY GPLGMTGISM GGHMASLAVS NWPKPMPLIP CLSWSTASGV FTTTDSFKMG QEFVKHFTSS ADKLTNLNLV SRTLNLDISN QVVSQKPADC HNSSKTSVSA TSEGLLLQDT SKMKRFNQTL STNKSGYTSR NPQSYHLLSK EQSRNSLRKE SLIFMKGVMD ECTHVANFSV PVDPSLIIVV QAKEDAYIPR TGVRSLQEIW PGCEIRYLEG GHISAYLFKQ GLFR |
| 预测分子量 | 46,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的模拟参考文献示例(请注意，ABHD18的研究可能较为有限，以下内容为虚构示例，供参考)：
1. **文献名称**: "Functional Characterization of ABHD18 as a Novel Lipase in Lipid Metabolism"
**作者**: Zhang L, et al.
**摘要**: 本研究首次报道了ABHD18重组蛋白的酶活性，证实其具有水解甘油三酯的能力，并可能通过调节脂滴动态影响细胞脂质储存。通过体外重组表达和质谱分析，作者鉴定了其催化三联体结构，提示其在代谢疾病中的潜在作用。
2. **文献名称**: "ABHD18 Interacts with Mitochondrial Proteins and Modulates Oxidative Stress"
**作者**: Patel R, et al.
**摘要**: 文章利用重组ABHD18蛋白进行免疫共沉淀实验，发现其与线粒体膜蛋白相互作用，可能参与调控ROS(活性氧)水平。敲低ABHD18导致细胞氧化应激加剧，提示其在线粒体功能中的新角色。
3. **文献名称**: "Structural Insights into ABHD18 via Recombinant Protein Crystallography"
**作者**: Kim S, et al.
**摘要**: 通过重组表达人源ABHD18蛋白并进行X射线晶体学分析，揭示了其α/β水解酶结构域的独特构象，为设计针对该酶的小分子抑制剂提供了结构基础，可能用于神经退行性疾病研究。
4. **文献名称**: "ABHD18 Expression Correlates with Cancer Cell Invasion in Glioblastoma"
**作者**: Chen X, et al.
**摘要**: 该研究在胶质母细胞瘤模型中检测到ABHD18重组蛋白的高表达，体外实验表明其通过调控鞘脂代谢促进肿瘤细胞迁移，提示其作为癌症治疗靶点的可能性。
---
**注意**：以上内容为模拟生成，ABHD18的实际研究可能较少，建议通过 **PubMed/Google Scholar** 结合关键词 "ABHD18 recombinant" 或 "ABHD18 function" 检索最新文献。若需具体文献协助，可提供更多背景信息。
ABHD18 (Alpha/Beta-Hydrolase Domain-Containing Protein 18) is a member of the serine hydrolase superfamily, characterized by a conserved α/β-hydrolase fold. This structural motif is shared across diverse enzymes, including lipases, esterases, and metabolic regulators. While the precise biological function of ABHD18 remains under investigation, its enzymatic activity is hypothesized to involve lipid metabolism or signaling pathways due to its structural homology with lipid-processing enzymes. Recent studies suggest potential roles in cellular processes such as membrane remodeling, energy homeostasis, or interorganelle communication.
Recombinant ABHD18 protein is typically produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to enable functional and structural studies. Its production allows researchers to characterize substrate specificity, catalytic mechanisms, and inhibitor interactions through biochemical assays. Structural analyses using X-ray crystallography or cryo-EM could reveal insights into its active site architecture and regulatory domains.
Emerging evidence links ABHD family proteins to human diseases, including metabolic disorders, cancer, and neurological conditions. While ABHD18's pathophysiological relevance remains less defined compared to well-studied relatives like ABHD6 (endocannabinoid hydrolase) or ABHD12 (phospholipase), its recombinant form serves as a critical tool for uncovering potential therapeutic targets. Challenges include elucidating endogenous substrates and validating physiological contexts for its activity. Current research focuses on mapping tissue distribution, post-translational modifications, and interactome networks to contextualize its cellular functions.
×
