| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | hmt2 |
| Uniprot No | O94284 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-459aa |
| 氨基酸序列 | ASTHHKVLVVGGGSAGISVAHQIYNKFSKYRFANDQGKDTSLKPGEIGIVDGAKYHYYQPGWTLTGAGLSSVAKTRRELASLVPADKFKLHPEFVKSLHPRENKIVTQSGQEISYDYLVMAAGIYTDFGRIKGLTEALDDPNTPVVTIYSEKYADAVYPWIEKTKSGNAIFTQPSGVLKCAGAPQKIMWMAEDYWRRHKVRSNIDVSFYTGMPTLFSVKRYSDALLRQNEQLHRNVKINYKDELVEVKGSERKAVFKNLNDGSLFERPFDLLHAVPSMRSHEFIAKSDLADKSGFVAVDQSTTQSTKFPNVFAIGDCSGLPTSKTYAAITAQAPVMVHNLWSFVNGKNLTASYNGYTSCPLLTGYGKLILAEFLYKQEPKESFGRFSRFLDQTVPRRMFYHLKKDFFPFVYWNFAVRNGKWYGSRGLIPPHFPVN |
| 预测分子量 | 51.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMT2重组蛋白的3篇假设性参考文献示例(注：文献为虚构，仅供格式参考，建议通过学术数据库核实具体研究)：
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1. **文献名称**：*Cloning and Characterization of Recombinant Hmt2 Protein in Schizosaccharomyces pombe*
**作者**：Kumar A., Smith P., et al.
**摘要**：本研究报道了裂殖酵母中Hmt2蛋白的重组表达与功能分析。Hmt2被鉴定为一种依赖S-腺苷甲硫氨酸的谷胱甘肽合成酶，重组蛋白通过大肠杆菌系统成功表达，并验证了其在氧化应激响应中的关键作用。
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2. **文献名称**：*Structural Analysis of Human HMT2 Reveals Substrate Specificity in Histone Methylation*
**作者**：Li J., Zhang Y., et al.
**摘要**：通过重组表达人源HMT2(组蛋白甲基转移酶同源蛋白2)，解析了其晶体结构，并发现其对组蛋白H3K4的特异性甲基化修饰功能，为表观遗传调控机制提供了新见解。
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3. **文献名称**：*Heterologous Expression of Plant HMT2 in Pichia pastoris for Biotechnological Applications*
**作者**：Wang L., Chen R., et al.
**摘要**：研究利用毕赤酵母系统高效表达植物来源的HMT2重组蛋白，优化了纯化工艺，并证明其在体外具有催化S-甲基转移活性，潜在应用于代谢工程领域。
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**提示**：若需真实文献，请使用关键词“HMT2 recombinant protein”“Hmt2 gene expression”在PubMed或Web of Science检索，并注意区分物种(如人、酵母、植物)及功能(如甲基转移酶、抗氧化酶)。
The Hmt2 recombinant protein is a genetically engineered variant derived from the Schizosaccharomyces pombe (fission yeast) Hmt2 gene, which encodes a key enzyme involved in cellular detoxification processes. Originally identified for its role in heavy metal tolerance, native Hmt2 functions as a glutathione-dependent formaldehyde dehydrogenase (GSH-FDH), catalyzing the oxidation of formaldehyde to formate using glutathione (GSH) as a cofactor. This activity links Hmt2 to the detoxification of reactive aldehydes and the maintenance of redox homeostasis, particularly under stress conditions.
Recombinant Hmt2 is produced via heterologous expression systems, such as Escherichia coli or yeast, enabling large-scale purification for biochemical and biomedical studies. Its production leverages cloning techniques to insert the hmt2 gene into expression vectors, followed by affinity chromatography for protein isolation. Researchers prioritize this recombinant form due to its solubility, stability, and retained enzymatic activity, which facilitates structural and functional analyses.
Interest in Hmt2 stems from its dual role in formaldehyde metabolism and oxidative stress response, with implications for understanding cellular defense mechanisms against environmental toxins. Additionally, studies explore its potential applications in bioremediation, biosensor development for aldehyde detection, and therapeutic strategies targeting oxidative damage in diseases like cancer or neurodegenerative disorders. Recent structural studies using recombinant Hmt2 have also shed light on substrate-binding mechanisms and catalytic residues, advancing enzyme engineering efforts. Ongoing research aims to optimize its industrial scalability and therapeutic efficacy, positioning Hmt2 as a versatile tool in both basic science and applied biotechnology.
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