纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | HNRNPH2 |
Uniprot No | P55795 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 37-302aa |
氨基酸序列 | QNGTSGIRFIYTREGRPSGEAFVELESEEEVKLALKKDRETMGHRYVEVFKSNSVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGMTLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYGGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSSFQSTTGHCVHMRGLPYRATE |
预测分子量 | 30 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的3-4篇关于HNRNPH2重组蛋白的参考文献示例(内容基于典型研究方向虚构,实际文献需通过数据库检索):
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1. **文献名称**: *"Recombinant HNRNPH2 Protein Production and Purification in E. coli for Functional Studies"*
**作者**: Smith A, et al.
**摘要**: 本研究描述了在大肠杆菌中高效表达和纯化重组HNRNPH2蛋白的方法,通过优化密码子使用和纯化步骤获得高纯度蛋白,并验证其RNA结合活性,为后续体外功能研究提供基础。
2. **文献名称**: *"Structural Insights into the RNA-Binding Domains of HNRNPH2 via X-ray Crystallography"*
**作者**: Chen L, et al.
**摘要**: 利用X射线晶体学解析了HNRNPH2蛋白的RNA结合结构域三维结构,揭示了其与特定RNA序列结合的分子机制,为理解HNRNPH2在剪接调控中的作用提供结构基础。
3. **文献名称**: *"HNRNPH2 Mutations Disrupt mRNA Splicing and Cause Neurodevelopmental Disorders: Evidence from Recombinant Protein Assays"*
**作者**: Johnson R, et al.
**摘要**: 通过构建携带患者突变位点的重组HNRNPH2蛋白,发现突变体丧失结合靶mRNA的能力,导致剪接异常,解释了HNRNPH2突变引发X连锁神经发育障碍的分子病理机制。
4. **文献名称**: *"HNRNPH2 Recombinant Protein Interactome Analysis Reveals Roles in Cancer Metastasis"*
**作者**: Wang Y, et al.
**摘要**: 基于重组HNRNPH2蛋白的免疫共沉淀-质谱分析,鉴定出其在癌细胞中与促转移因子的相互作用网络,提示HNRNPH2可能通过调控特定mRNA稳定性参与肿瘤侵袭过程。
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注:以上文献为示例性质,实际研究中需通过PubMed、Web of Science等平台检索真实文献(关键词:HNRNPH2 recombinant, HNRNPH2 gene expression)。如需具体文献协助,可提供更详细的研究方向。
The heterogeneous nuclear ribonucleoprotein H2 (HNRNPH2) is a member of the HNRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, transport, stability, and translation regulation. HNRNPH2 specifically binds to RNA through its RNA recognition motifs (RRMs), facilitating interactions with GU-rich sequences or splice site elements. It is involved in alternative splicing decisions, influencing the diversity of mRNA isoforms and protein products. Dysregulation of HNRNPH2 has been linked to neurodevelopmental disorders, such as X-linked intellectual disability, and certain cancers, highlighting its importance in cellular homeostasis and disease.
Recombinant HNRNPH2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce functional, purified protein for *in vitro* studies. Its recombinant form enables detailed biochemical and structural analyses, such as elucidating RNA-binding specificity, mapping interaction domains, or screening for small-molecule modulators. Researchers utilize affinity tags (e.g., His-tag) for efficient purification and detection. Studies leveraging recombinant HNRNPH2 have advanced understanding of its role in RNA processing mechanisms and pathogenicity of mutations. Additionally, it serves as a tool for developing therapeutic strategies targeting splicing defects or HNRNPH2-associated pathways. The protein’s conserved functional domains and disease relevance make it a focal point in both basic and translational research.
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