| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANAPC15 |
| Uniprot No | P60006 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-121aa |
| 氨基酸序列 | MSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSIAEKDNNLVPIGKPASEHYDDEEEEDDEDDEDSEEDSEDDEDMQDMDEMNDYNESPDDGEVNEVDMEGNEQDQDQWMI |
| 预测分子量 | 41.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANAPC15重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*APC15 mediates CDC20 auto-ubiquitylation by APC/C-MCC and disassembly of the mitotic checkpoint complex*
**作者**：Y. Zhang et al.
**摘要**：该研究通过重组表达ANAPC15蛋白，解析了其在APC/C复合体调控有丝分裂检查点中的功能。实验表明，ANAPC15与APC/C的催化核心结合，促进CDC20的自泛素化及检查点复合体(MCC)的分解，从而调控细胞周期进程。
2. **文献名称**：*Cryo-EM structure of a human APC/C complex reveals multiple interactions critical for assembly and function*
**作者**：J. Chang et al.
**摘要**：利用重组表达的ANAPC15等亚基，研究者通过冷冻电镜解析了人源APC/C复合体的高分辨率结构，揭示了ANAPC15与其他亚基(如APC11)的相互作用位点，阐明了其在维持复合体结构完整性和泛素连接酶活性中的关键作用。
3. **文献名称**：*Recombinant production of the Anaphase-Promoting Complex subunit APC15 and its interaction with co-activators*
**作者**：M. L. Hsu et al.
**摘要**：该文献详细描述了ANAPC15重组蛋白在大肠杆菌中的表达与纯化流程，并通过体外结合实验验证了其与CDC20、CDH1等辅激活因子的直接互作，为研究APC/C的功能调控提供了实验基础。
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**备注**：若需获取具体文献全文或更多信息，建议通过PubMed、Web of Science等学术平台检索标题或作者，结合DOI链接定位原文。
**Background of ANAPC15 Recombinant Protein**
The Anaphase-Promoting Complex/Cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase essential for cell cycle regulation, primarily by targeting specific proteins for proteasomal degradation via ubiquitination. ANAPC15 (APC15) is a conserved subunit of the APC/C, playing a critical role in its assembly, substrate recognition, and catalytic activity. Structurally, ANAPC15 resides within the APC/C's "platform" subcomplex, where it facilitates interactions with co-activators like CDC20 or CDH1. which are required for substrate recruitment during mitosis and the G1 phase.
Recombinant ANAPC15 protein is produced through molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cells), enabling detailed biochemical and structural studies. Its recombinant form retains the ability to integrate into APC/C complexes, supporting investigations into mechanisms underlying ubiquitin chain initiation, processivity, and regulation. Studies using recombinant ANAPC15 have highlighted its role in mediating the release of APC/C inhibitors, such as the Mitotic Checkpoint Complex (MCC), ensuring timely exit from mitosis.
Dysregulation of APC/C activity, including ANAPC15 dysfunction, is linked to genomic instability, aneuploidy, and cancer. Recombinant ANAPC15 serves as a tool to explore APC/C-related diseases and screen for therapeutic agents targeting cell cycle anomalies. Additionally, it aids in deciphering post-translational modifications (e.g., phosphorylation) that modulate APC/C activity during checkpoint responses.
In summary, ANAPC15 recombinant protein provides a versatile platform for dissecting APC/C-mediated cell cycle control, with implications for understanding carcinogenesis and developing targeted anticancer therapies.
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