| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCM3AP |
| Uniprot No | O60318 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1980aa |
| 氨基酸序列 | MNPTNPFSGQQPSAFSASSSNVGTLPSKPPFRFGQPSLFGQNSTLSGKSSGFSQVSSFPASSGVSHSSSVQTLGFTQTSSVGPFSGLEHTSTFVATSGPSSSSVLGNTGFSFKSPTSVGAFPSTSAFGQEAGEIVNSGFGKTEFSFKPLENAVFKPILGAESEPEKTQSQIASGFFTFSHPISSAPGGLAPFSFPQVTSSSATTSNFTFSKPVSSNNSLSAFTPALSNQNVEEEKRGPKSIFGSSNNSFSSFPVSSAVLGEPFQASKAGVRQGCEEAVSQVEPLPSLMKGLKRKEDQDRSPRRHGHEPAEDSDPLSRGDHPPDKRPVRLNRPRGGTLFGRTIQDVFKSNKEVGRLGNKEAKKETGFVESAESDHMAIPGGNQSVLAPSRIPGVNKEEETESREKKEDSLRGTPARQSNRSESTDSLGGLSPSEVTAIQCKNIPDYLNDRTILENHFGKIAKVQRIFTRRSKKLAVVHFFDHASAALARKKGKSLHKDMAIFWHRKKISPNKKPFSLKEKKPGDGEVSPSTEDAPFQHSPLGKAAGRTGASSLLNKSSPVKKPSLLKAHQFEGDSFDSASEGSEGLGPCVLSLSTLIGTVAETSKEKYRLLDQRDRIMRQARVKRTDLDKARTFVGTCLDMCPEKERYMRETRSQLSVFEVVPGTDQVDHAAAVKEYSRSSADQEEPLPHELRPLPVLSRTMDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTVSLIEKCTRFHIHCAHFMCEEPMSSFDAKINNENMTKCLQSLKEMYQDLRNKGVFCASEAEFQGYNVLLSLNKGDILREVQQFHPAVRNSSEVKFAVQAFAALNSNNFVRFFKLVQSASYLNACLLHCYFSQIRKDALRALNFAYTVSTQRSTIFPLDGVVRMLLFRDCEEATDFLTCHGLTVSDGCVELNRSAFLEPEGLSKTRKSVFITRKLTVSVGEIVNGGPLPPVPRHTPVCSFNSQNKYIGESLAAELPVSTQRPGSDTVGGGRGEECGVEPDAPLSSLPQSLPAPAPSPVPLPPVLALTPSVAPSLFQLSVQPEPPPPEPVPMYSDEDLAQVVDELIQEALQRDCEEVGSAGAAYAAAALGVSNAAMEDLLTAATTGILRHIAAEEVSKERERREQERQRAEEERLKQERELVLSELSQGLAVELMERVMMEFVRETCSQELKNAVETDQRVRVARCCEDVCAHLVDLFLVEEIFQTAKETLQELQCFCKYLQRWREAVTARKKLRRQMRAFPAAPCCVDVSDRLRALAPSAECPIAEENLARGLLDLGHAGRLGISCTRLRRLRNKTAHQMKVQHFYQQLLSDVAWASLDLPSLVAEHLPGRQEHVFWKLVLVLPDVEEQSPESCGRILANWLKVKFMGDEGSVDDTSSDAGGIQTLSLFNSLSSKGDQMISVNVCIKVAHGALSDGAIDAVETQKDLLGASGLMLLLPPKMKSEDMAEEDVYWLSALLQLKQLLQAKPFQPALPLVVLVPSPGGDAVEKEVEDGLMLQDLVSAKLISDYTVTEIPDTINDLQGSTKVLQAVQWLVSHCPHSLDLCCQTLIQYVEDGIGHEFSGRFFHDRRERRLGGLASQEPGAIIELFNSVLQFLASVVSSEQLCDLSWPVTEFAEAGGSRLLPHLHWNAPEHLAWLKQAVLGFQLPQMDLPPLGAPWLPVCSMVVQYASQIPSSRQTQPVLQSQVENLLHRTYCRWKSKSPSPVHGAGPSVMEIPWDDLIALCINHKLRDWTPPRLPVTSEALSEDGQICVYFFKNDLKKYDVPLSWEQARLQTQKELQLREGRLAIKPFHPSANNFPIPLLHMHRNWKRSTECAQEGRIPSTEDLMRGASAEELLAQCLSSSLLLEKEENKRFEDQLQQWLSEDSGAFTDLTSLPLYLPQTLVSLSHTIEPVMKTSVTTSPQSDMMREQLQLSEATGTCLGERLKHLERLIRSSREEEVASELHLSALLDMVDI |
| 预测分子量 | 218 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCM3AP重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*MCM3AP, a novel acetyltransferase that acetylates the MCM3 protein in DNA replication initiation*
**作者**：Takei, Y., et al.
**摘要**：本研究通过重组蛋白技术表达并纯化了MCM3AP，发现其作为乙酰转移酶直接乙酰化MCM3蛋白，调控DNA复制起始过程。实验表明，乙酰化修饰影响MCM复合物的染色质结合能力，进而影响复制起始效率。
2. **文献名称**：*Overexpression of MCM3AP in hepatocellular carcinoma correlates with poor prognosis and promotes cell proliferation*
**作者**：Huang, J., et al.
**摘要**：通过重组MCM3AP蛋白的体外功能实验，作者发现其在肝细胞癌中异常高表达，并通过激活PI3K/AKT信号通路促进肿瘤细胞增殖。临床数据分析表明，MCM3AP过表达与患者预后不良显著相关。
3. **文献名称**：*MCM3AP interacts with replication protein A (RPA) and modulates DNA damage response*
**作者**：Yuan, Z., et al.
**摘要**：利用重组MCM3AP蛋白的体外结合实验，该研究揭示了其与DNA损伤关键蛋白RPA的相互作用。结果表明，MCM3AP通过调控RPA的磷酸化状态，影响细胞在复制压力下的DNA损伤修复能力。
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**备注**：以上文献信息为示例，实际引用时需核对具体作者、年份及期刊名称。如需进一步协助检索全文，建议通过PubMed或Web of Science等数据库查询关键词“MCM3AP recombinant protein”。
MCM3AP (Minichromosome Maintenance Complex Component 3 Associated Protein) is a multifunctional protein with critical roles in DNA replication and RNA export. Originally identified as an interacting partner of MCM3. a core component of the MCM helicase complex essential for DNA replication initiation, MCM3AP is evolutionarily conserved and ubiquitously expressed in eukaryotic cells. It functions as a acetyltransferase (AT) by acetylating MCM3. modulating helicase activity and ensuring proper replication fork progression. Beyond replication, MCM3AP also participates in mRNA nuclear export through its association with the mRNA export complex, highlighting its dual functionality in nucleic acid metabolism.
Structurally, MCM3AP contains an N-terminal acetyltransferase domain and a C-terminal region involved in protein-protein interactions. Its recombinant form is typically produced in bacterial or eukaryotic expression systems for biochemical and functional studies. Recombinant MCM3AP enables researchers to dissect its enzymatic activity, substrate specificity, and interactions with replication/export machinery components.
Dysregulation of MCM3AP is linked to human diseases. Germline mutations cause autosomal recessive primary microcephaly and intellectual disability, while altered expression correlates with cancer progression. In gliomas and hepatocellular carcinomas, MCM3AP overexpression promotes genomic instability and tumor growth, making it a potential therapeutic target. Recombinant MCM3AP proteins are instrumental in screening small-molecule inhibitors and developing strategies to counteract replication stress in malignancies. Current research also explores its role in viral infection responses, as some viruses exploit host replication-export systems. Further characterization of recombinant MCM3AP may unveil novel mechanisms in genome maintenance and RNA biology.
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