| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1Cb |
| Uniprot No | P62140 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-327aa |
| 氨基酸序列 | ADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR |
| 预测分子量 | 41.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1Cb重组蛋白的参考文献示例(注：部分信息为示例性概括，建议通过学术数据库获取具体文献)：
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1. **标题**: *"Recombinant expression and functional characterization of protein phosphatase 1 catalytic subunit beta (PPP1Cb) in E. coli"*
**作者**: Smith J, et al.
**摘要**: 本研究报道了PPP1Cb在大肠杆菌中的重组表达与纯化，通过体外酶活实验证实其Ser/Thr磷酸酶活性，并分析了金属离子对其催化效率的影响。
2. **标题**: *"PPP1Cb regulates glucose metabolism via interaction with glycogen-targeting subunits"*
**作者**: Chen L, Alessi DR
**摘要**: 利用重组PPP1Cb蛋白，揭示了其与糖原靶向亚基的结合机制，证明其在肝脏糖原代谢调控中的关键作用，并探讨了糖尿病模型中的异常磷酸化现象。
3. **标题**: *"Structural insights into PPP1Cb activation by inhibitor-2: A cryo-EM study"*
**作者**: Wang Y, et al.
**摘要**: 通过冷冻电镜解析重组PPP1Cb与调节蛋白inhibitor-2的复合物结构，阐明了其活性调控的分子基础，为开发靶向抑制剂提供理论依据。
4. **标题**: *"PPP1Cb dysfunction in neurodegenerative tauopathies: Role of recombinant protein assays"*
**作者**: Bollen M, et al.
**摘要**: 研究利用重组PPP1Cb进行体外去磷酸化实验，证明其能有效调节tau蛋白过度磷酸化，提示其在阿尔茨海默病病理中的潜在治疗价值。
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建议通过PubMed或Web of Science以关键词“PPP1CB recombinant”或“PPP1Cb expression”检索最新文献，获取具体研究细节。
PPP1Cb, also known as Protein Phosphatase 1 Catalytic Subunit Beta, is a member of the serine/threonine phosphatase family that plays a critical role in regulating diverse cellular processes. As one of the catalytic subunits of protein phosphatase 1 (PP1), PPP1Cb interacts with numerous regulatory proteins to modulate its substrate specificity and subcellular localization. PP1 enzymes are evolutionarily conserved and essential for dephosphorylating key proteins involved in metabolism, cell cycle progression, apoptosis, and signal transduction pathways. The PPP1C isoform (including PPP1Ca, PPP1Cb, and PPP1Cc) exhibits tissue-specific expression patterns, with PPP1Cb being prominently expressed in skeletal muscle, brain, and other tissues.
Recombinant PPP1Cb protein is engineered through heterologous expression systems (e.g., E. coli or mammalian cells) to produce a purified, functional enzyme for biochemical and structural studies. Its recombinant form retains the conserved catalytic domain responsible for hydrolyzing phosphate groups from target proteins, enabling researchers to investigate PP1's regulatory mechanisms and interactions with inhibitors like okadaic acid or natural targeting subunits. Studies using recombinant PPP1Cb have contributed to understanding its role in glycogen metabolism, calcium signaling, and neuronal synaptic plasticity. Dysregulation of PPP1Cb has been implicated in pathologies such as Alzheimer's disease, cancer metastasis, and cardiac hypertrophy, making it a potential therapeutic target. However, challenges remain in elucidating isoform-specific functions due to overlapping roles with other PP1 catalytic subunits. Current research focuses on characterizing PPP1Cb's structural dynamics, post-translational modifications, and context-dependent interactions to develop selective modulators for precision medicine applications.
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