| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMP34 |
| Uniprot No | O43808 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-307aa |
| 氨基酸序列 | MASVLSYESLVHAVAGAVGSVTAMTVFFPLDTARLRLQVDEKRKSKTTHMVLLEIIKEEGLLAPYRGWFPVISSLCCSNFVYFYTFNSLKALWVKGQHSTTGKDLVVGFVAGVVNVLLTTPLWVVNTRLKLQGAKFRNEDIVPTNYKGIIDAFHQIIRDEGISALWNGTFPSLLLVFNPAIQFMFYEGLKRQLLKKRMKLSSLDVFIIGAVAKAIATTVTYPLQTVQSILRFGRHRLNPENRTLGSLRNILYLLHQRVRRFGIMGLYKGLEAKLLQTVLTAALMFLVYEKLTAATFTVMGLKRAHQH |
| 预测分子量 | 34,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PMP34(线粒体载体蛋白相关)重组蛋白研究的3篇文献示例(部分信息为模拟概括，实际文献需通过数据库验证)：
1. **文献名称**：*Functional characterization of the human PMP34. a peroxisomal transporter of coenzyme A and adenosine 3',5'-diphosphate*
**作者**：Agrimi, G. et al.
**摘要**：本研究通过重组表达人源PMP34蛋白，证实其作为过氧化物酶体膜上的转运体，负责辅酶A和3',5'-二磷酸腺苷的跨膜运输，揭示了其在脂肪酸β氧化代谢中的关键作用。
2. **文献名称**：*The mitochondrial carrier family protein PMP34 regulates cellular energy metabolism in cancer cells*
**作者**：Yamashita, T. et al.
**摘要**：文章利用重组PMP34蛋白进行功能分析，发现其在肿瘤细胞线粒体中通过调控丙酮酸转运影响能量代谢，并证明其高表达与癌细胞增殖和化疗耐药性相关。
3. **文献名称**：*Structural insights into the substrate specificity of PMP34 through cryo-EM and molecular dynamics simulations*
**作者**：Chen, L. et al.
**摘要**：通过重组PMP34蛋白的冷冻电镜结构与分子动力学模拟，揭示了该蛋白底物结合位点的三维构象，阐明了其选择性转运短链脂肪酸衍生物的分子机制。
**注意**：以上文献为示例性概括，实际研究中需通过PubMed、Web of Science等数据库以“PMP34 recombinant protein”“SLC25A17”(可能的基因名)等关键词检索真实文献。若研究领域涉及植物或微生物，需补充对应物种信息。
**Background of PMP34 Recombinant Protein**
PMP34 (Peroxisomal Membrane Protein 34), also known as *SLC25A17* in humans, is a member of the mitochondrial carrier family (MCF) of proteins. It is primarily localized to the peroxisomal membrane, where it facilitates the transport of small molecules, including ATP, coenzyme A, and other nucleotides, across the membrane. This transport activity is critical for maintaining peroxisomal metabolic functions, such as fatty acid β-oxidation, bile acid synthesis, and detoxification of reactive oxygen species.
Structurally, PMP34 contains six transmembrane domains and characteristic MCF motifs, which enable its role as a solute carrier. Dysregulation of PMP34 has been linked to peroxisomal disorders, including Zellweger spectrum disorders, where impaired peroxisomal function leads to severe metabolic and neurological abnormalities.
Recombinant PMP34 protein is engineered using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography. Its production enables functional studies, such as substrate specificity assays, structural analysis (e.g., X-ray crystallography or cryo-EM), and investigations into peroxisome biogenesis. Additionally, recombinant PMP34 serves as a tool for developing therapeutic strategies targeting peroxisomal diseases or metabolic syndromes. Research also explores its potential role in cancer, where altered peroxisomal metabolism may influence tumor progression.
Overall, PMP34 recombinant protein is a vital resource for deciphering peroxisome-related biochemistry and advancing treatments for associated disorders.
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