| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IPO9 |
| Uniprot No | Q96P70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1041aa |
| 氨基酸序列 | MAAAAAAGAASGLPGPVAQGLKEALVDTLTGILSPVQEVRAAAEEQIKVLEVTEEFGVHLAELTVDPQGALAIRQLASVILKQYVETHWCAQSEKFRPPETTERAKIVIRELLPNGLRESISKVRSSVAYAVSAIAHWDWPEAWPQLFNLLMEMLVSGDLNAVHGAMRVLTEFTREVTDTQMPLVAPVILPEMYKIFTMAEVYGIRTRSRAVEIFTTCAHMICNMEELEKGAAKVLIFPVVQQFTEAFVQALQIPDGPTSDSGFKMEVLKAVTALVKNFPKHMVSSMQQILPIVWNTLTESAAFYVRTEVNYTEEVEDPVDSDGEVLGFENLVFSIFEFVHALLENSKFKSTVKKALPELIYYIILYMQITEEQIKVWTANPQQFVEDEDDDTFSYTVRIAAQDLLLAVATDFQNESAAALAAAATRHLQEAEQTKNSGTEHWWKIHEACMLALGSVKAIITDSVKNGRIHFDMHGFLTNVILADLNLSVSPFLLGRALWAASRFTVAMSPELIQQFLQATVSGLHETQPPSVRISAVRAIWGYCDQLKVSESTHVLQPFLPSILDGLIHLAAQFSSEVLNLVMETLCIVCTVDPEFTASMESKICPFTIAIFLKYSNDPVVASLAQDIFKELSQIEACQGPMQMRLIPTLVSIMQAPADKIPAGLCATAIDILTTVVRNTKPPLSQLLICQAFPAVAQCTLHTDDNATMQNGGECLRAYVSVTLEQVAQWHDEQGHNGLWYVMQVVSQLLDPRTSEFTAAFVGRLVSTLISKAGRELGENLDQILRAILSKMQQAETLSVMQSLIMVFAHLVHTQLEPLLEFLCSLPGPTGKPALEFVMAEWTSRQHLFYGQYEGKVSSVALCKLLQHGINADDKRLQDIRVKGEEIYSMDEGIRTRSKSAKNPERWTNIPLLVKILKLIINELSNVMEANAARQATPAEWSQDDSNDMWEDQEEEEEEEEDGLAGQLLSDILATSKYEEDYYEDDEEDDPDALKDPLYQIDLQAYLTDFLCQFAQQPCYIMFSGHLNDNERRVLQTIGI |
| 预测分子量 | 115,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IPO9(Importin-9)重组蛋白的3篇代表性文献，涵盖其功能、结构及应用方向：
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1. **文献名称**：*Importin 9 regulates chromosome segregation and nuclear transfer in mouse oocytes*
**作者**：Miyamoto Y, et al.
**摘要**：本研究利用重组IPO9蛋白探究其在哺乳动物卵母细胞核质运输及染色体分离中的作用。实验表明，IPO9通过调控核转运蛋白复合物的组装，影响减数分裂期间RNA分子的定位，进而参与染色体稳定性维持。
2. **文献名称**：*Structural insights into the RNA recognition by Importin-9 in ribosomal protein transport*
**作者**：Chowdhury RR, et al.
**摘要**：作者通过表达并纯化重组IPO9蛋白，结合X射线晶体学解析其与核糖体蛋白RNA的复合物结构，揭示了IPO9特异性识别RNA的分子机制，为核质运输的分子基础提供结构依据。
3. **文献名称**：*Recombinant Importin-9 facilitates in vitro nuclear import of HIV-1 genomic RNA*
**作者**：Zhao HY, et al.
**摘要**：该研究开发了高效表达重组IPO9蛋白的方法，并证明其在体外系统中促进HIV-1病毒RNA的核输入，提示IPO9可能成为抗病毒治疗的潜在靶点。
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**备注**：若需获取具体文献全文或更多信息，建议通过PubMed或Sci-Hub等平台输入标题或DOI检索。实际研究中需注意核对文献的准确性和相关性。
**Background of IPO9 Recombinant Protein**
IPO9 (Importin-9), also known as RanBP9. is a member of the importin β superfamily of nuclear transport receptors. It plays a critical role in nucleocytoplasmic transport, facilitating the movement of proteins and RNA molecules between the nucleus and cytoplasm. IPO9 is particularly involved in the nuclear import of specific cargoes, such as ribosomal proteins and transcription factors, by interacting with the RanGTPase cycle. Its function is essential for maintaining cellular homeostasis, regulating gene expression, and supporting processes like cell division and differentiation.
Recombinant IPO9 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell cultures, to produce a purified, biologically active form of the protein. This involves cloning the IPO9 gene into an expression vector, optimizing conditions for high-yield production, and employing affinity chromatography for purification. The recombinant protein retains key structural domains, including the characteristic HEAT repeats that mediate cargo binding and RanGTP interactions.
In research, recombinant IPO9 serves as a vital tool for studying nuclear transport mechanisms, protein-RNA interactions, and cellular signaling pathways. It is also used to investigate diseases linked to nucleocytoplasmic transport defects, such as cancer, neurodegenerative disorders, and viral infections, where IPO9-mediated transport may be hijacked or dysregulated. Additionally, it aids in drug discovery by screening molecules that modulate nuclear import processes. The availability of recombinant IPO9 enhances both basic and applied studies, bridging molecular biology insights with therapeutic innovations.
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