Recombinant Human DIC protein

中文名： 二羧酸转运蛋白(DIC)重组蛋白
别名： DIC；DIC；Mitochondrial dicarboxylate carrier

货号: PA2000-1220

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	DIC
Uniprot No	Q9UBX3
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-287aa
氨基酸序列	MAAEARVSRWYFGGLASCGAACCTHPLDLLKVHLQTQQEVKLRMTGMALRVVRTDGILALYSGLSASLCRQMTYSLTRFAIYETVRDRVAKGSQGPLPFHEKVLLGSVSGLAGGFVGTPADLVNVRMQNDVKLPQGQRRNYAHALDGLYRVAREEGLRRLFSGATMASSRGALVTVGQLSCYDQAKQLVLSTGYLSDNIFTHFVASFIAGGCATFLCQPLDVLKTRLMNSKGEYQGVFHCAVETAKLGPLAFYKGLVPAGIRLIPHTVLTFVFLEQLRKNFGIKVPS
预测分子量	31,2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于DIC(假设为弥散性血管内凝血)相关重组蛋白的示例参考文献，内容为模拟生成，仅供参考：

1. **文献名称**：*Recombinant Human Thrombomodulin in the Treatment of DIC: A Multicenter Trial*

**作者**：Saito H, et al.

**摘要**：评估重组人血栓调节蛋白(rTM)治疗DIC的安全性和有效性，临床数据显示其显著改善凝血参数和器官功能，降低死亡率。

2. **文献名称**：*Expression and Purification of Recombinant Protein C for Anticoagulant Therapy in DIC*

**作者**：Grey ST, et al.

**摘要**：报道重组蛋白C在哺乳动物细胞中的高效表达与纯化方法，并验证其在DIC模型中的抗凝和抗炎作用。

3. **文献名称**：*Engineering a Stable Recombinant Factor VIIa Variant for DIC Management*

**作者**：Petersen LC, et al.

**摘要**：通过基因工程改造重组凝血因子VIIa，增强其稳定性与活性，动物实验表明其可有效纠正DIC相关的出血和血栓失衡。

4. **文献名称**：*Role of Recombinant ADAMTS13 in Thrombotic Microangiopathy-Associated DIC*

**作者**：Zheng XL, et al.

**摘要**：研究重组ADAMTS13蛋白对血栓性微血管病诱发DIC的治疗潜力，证实其通过调控血管性血友病因子多聚体改善微循环障碍。

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**提示**：实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“recombinant protein”、“DIC treatment”、“thrombomodulin”、“protein C”等获取。部分研究可能聚焦特定重组凝血因子或抗凝蛋白在DIC中的机制与应用。

背景信息

**Background of Recomcombinant Proteins**

Recombinant proteins are engineered through genetic modification, where target protein-encoding genes are inserted into host organisms (e.g., *E. coli*, yeast, or mammalian cells) for expression. This technology, rooted in recombinant DNA breakthroughs of the 1970s, revolutionized biopharmaceuticals and research by enabling large-scale production of proteins with precise sequences.

Key applications include therapeutic agents (e.g., insulin, monoclonal antibodies), diagnostic tools, and industrial enzymes. Recombinant methods overcome limitations of natural extraction, such as low yield, contamination risks, and ethical concerns. For example, human growth hormone, once isolated from cadavers, is now sustainably produced via bacterial systems.

Production involves cloning the gene into an expression vector, optimizing host conditions for folding/post-translational modifications (e.g., glycosylation in CHO cells), and purification via chromatography. Despite advancements, challenges persist, including protein aggregation, incorrect folding, and host-specific impurities. Innovations like codon optimization, fusion tags (e.g., His-tag), and high-throughput screening address these issues.

Recent trends focus on *in silico* design, CRISPR-edited cell lines, and continuous bioprocessing to enhance efficiency. Recombinant proteins also underpin cutting-edge therapies like CAR-T cells and mRNA vaccines, highlighting their pivotal role in modern medicine and biotechnology.

In summary, recombinant protein technology bridges molecular biology and industrial application, driving innovations across healthcare, agriculture, and bioengineering while continually evolving to meet emerging scientific and societal demands. (Word count: 245)

*Note: The term "DIC" was interpreted as a potential typo or context-specific abbreviation. The response focuses on general recombinant protein background. Clarification of "DIC" could refine the answer further.*