| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAB2 |
| Uniprot No | P98082 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-770aa |
| 氨基酸序列 | MSNEVETSATNGQPDQQAAPKAPSKKEKKKGPEKTDEYLLARFKGDGVKY KAKLIGIDDVPDARGDKMSQDSMMKLKGMAAAGRSQGQHKQRIWVNISLS GIKIIDEKTGVIEHEHPVNKISFIARDVTDNRAFGYVCGGEGQHQFFAIK TGQQAEPLVVDLKDLFQVIYNVKKKEEEKKKIEEASKAVENGSEALMILD DQTNKLKSGVDQMDLFGDMSTPPDLNSPTESKDILLVDLNSEIDTNQNSL RENPFLTNGITSCSLPRPTPQASFLPENAFSANLNFFPTPNPDPFRDDPF TQPDQSTPSSFDSLKSPDQKKENSSSSSTPLSNGPLNGDVDYFGQQFDQI SNRTGKQEAQAGPWPFSSSQTQPAVRTQNGVSEREQNGFSVKSSPNPFVG SPPKGLSIQNGVKQDLESSVQSSPHDSIAIIPPPQSTKPGRGRRTAKSSA NDLLASDIFAPPVSEPSGQASPTGQPTALQPNPLDLFKTSAPAPVGPLVG LGGVTVTLPQAGPWNTASLVFNQSPSMAPGAMMGGQPSGFSQPVIFGTSP AVSGWNQPSPFAASTPPPVPVVWGPSASVAPNAWSTTSPLGNPFQSNIFP APAVSTQPPSMHSSLLVTPPQPPPRAGPPKDISSDAFTALDPLGDKEIKD VKEMFKDFQLWQPPAVPARKGEQTSSGTLSAFASYFNSKVGIPQENADHD DFDANQLLNKINEPPKPAPRQVSLPVTKSTDNAFENPFFKDSFGSSQASV ASSQPVSSEMYRDPFGNPFA |
| 预测分子量 | 110 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DAB2重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
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1. **文献名称**：Expression and Purification of Recombinant DAB2 Protein in Escherichia coli
**作者**：Smith A, et al.
**摘要**：本研究报道了利用大肠杆菌表达系统高效表达人源DAB2重组蛋白的优化方法，通过His标签亲和层析纯化获得高纯度蛋白，并验证其与AP2复合物的体外结合活性，为后续功能研究提供工具。
2. **文献名称**：DAB2 Recombinant Protein Inhibits TGF-β Signaling in Ovarian Cancer Cells
**作者**：Chen L, et al.
**摘要**：作者通过哺乳动物细胞表达系统制备DAB2重组蛋白，发现其可竞争性抑制TGF-β受体信号通路，降低卵巢癌细胞迁移能力，提示DAB2在肿瘤治疗中的潜在应用价值。
3. **文献名称**：Structural Analysis of DAB2 PTB Domain Using Recombinant Protein Crystallography
**作者**：Wang X, et al.
**摘要**：本研究解析了DAB2重组蛋白PTB结构域的晶体结构，揭示了其与磷脂酰肌醇结合的关键氨基酸位点，为理解DAB2在内吞作用中的分子机制提供结构基础。
4. **文献名称**：Functional Characterization of Recombinant DAB2 in Clathrin-Mediated Endocytosis
**作者**：Johnson R, et al.
**摘要**：通过体外重建实验证明，纯化的DAB2重组蛋白能够促进网格蛋白包被囊泡的形成，并增强LDL受体内吞效率，证实DAB2在细胞膜运输中的关键作用。
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注：以上文献信息为模拟内容，实际引用时请通过PubMed、Web of Science等数据库检索真实文献。
DAB2 (Disabled-2) is a multifunctional adaptor protein encoded by the *DAB2* gene, playing critical roles in cellular signaling, endocytosis, and tissue morphogenesis. Initially identified as a regulator of cell adhesion and migration, DAB2 interacts with components of the TGF-β, Wnt, and Notch signaling pathways, modulating their activity. It contains a phosphotyrosine-binding (PTB) domain that binds to membrane receptors (e.g., LDL receptors) and a proline-rich C-terminal region involved in protein-protein interactions. DAB2 acts as a tumor suppressor, frequently downregulated in cancers (e.g., ovarian, breast) due to promoter hypermethylation, contributing to uncontrolled proliferation and metastasis.
Recombinant DAB2 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) for functional studies. Purified using affinity tags (His, GST), it enables *in vitro* investigations into its role in clathrin-mediated endocytosis, receptor trafficking, and signaling crosstalk. Researchers utilize it to explore mechanisms underlying epithelial-mesenchymal transition (EMT), cell polarity, and therapeutic strategies targeting DAB2 loss in cancer. Its structural domains are also studied to design peptide inhibitors or mimetics for modulating disease-related pathways. As a tool, recombinant DAB2 aids in elucidating its dual role in development and pathology, bridging basic research and translational applications.
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