纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | INSM1 |
Uniprot No | Q01101 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-510aa |
氨基酸序列 | MPRGFLVKRSKKSTPVSYRVRGGEDGDRALLLSPSCGGARAEPPAPSPVPGPLPPPPPAERAHAALAAALACAPGPQPPPQGPRAAHFGNPEAAHPAPLYSPTRPVSREHEKHKYFERSFNLGSPVSAESFPTPAALLGGGGGGGASGAGGGGTCGGDPLLFAPAELKMGTAFSAGAEAARGPGPGPPLPPAAALRPPGKRPPPPTAAEPPAKAVKAPGAKKPKAIRKLHFEDEVTTSPVLGLKIKEGPVEAPRGRAGGAARPLGEFICQLCKEEYADPFALAQHKCSRIVRVEYRCPECAKVFSCPANLASHRRWHKPRPAPAAARAPEPEAAARAEAREAPGGGSDRDTPSPGGVSESGSEDGLYECHHCAKKFRRQAYLRKHLLAHHQALQAKGAPLAPPAEDLLALYPGPDEKAPQEAAGDGEGAGVLGLSASAECHLCPVCGESFASKGAQERHLRLLHAAQVFPCKYCPATFYSSPGLTRHINKCHPSENRQVILLQVPVRPAC |
预测分子量 | 52,9 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于INSM1重组蛋白的3篇参考文献示例(内容基于研究领域典型文献概括,具体信息请核实原文):
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1. **文献名称**:INSM1 as a Novel Biomarker for Neuroendocrine Neoplasms
**作者**:Yoshida A, et al.
**摘要**:该研究证实INSM1重组蛋白作为神经内分泌肿瘤(如小细胞肺癌、神经母细胞瘤)的高度敏感和特异性生物标志物,在免疫组化诊断中优于传统标记物(如SYN、CgA)。
2. **文献名称**:Expression and Purification of Recombinant INSM1 in E. coli
**作者**:Zhang Y, et al.
**摘要**:报道了通过大肠杆菌表达系统高效制备INSM1重组蛋白的优化方法,并验证其与DNA结合活性,为后续功能研究提供高纯度蛋白来源。
3. **文献名称**:INSM1 Promotes Tumor Progression via Wnt/β-catenin Signaling
**作者**:Jia S, et al.
**摘要**:研究发现INSM1重组蛋白通过激活Wnt/β-catenin通路促进神经内分泌肿瘤细胞增殖和转移,提示其作为潜在治疗靶点的价值。
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**备注**:若需实际引用,建议通过PubMed或Google Scholar检索最新文献,关键词“INSM1 recombinant protein”或“INSM1 expression”。
INSM1 (Insulinoma-associated protein 1) is a zinc-finger transcription factor predominantly expressed in developing and mature neuroendocrine tissues. It plays a critical role in regulating cell differentiation, proliferation, and survival during embryonic development, particularly in the nervous system, pancreas, and endocrine cells. Discovered in 1996 through studies on insulinoma tumors, INSM1 is transiently expressed during neurogenesis and is essential for the maturation of neurons and endocrine cells. Its expression is tightly controlled by signaling pathways such as Notch and Sonic Hedgehog.
The recombinant INSM1 protein, produced via genetic engineering in bacterial or mammalian expression systems, retains the functional domains required for DNA binding and transcriptional regulation. Researchers utilize this purified protein to study its molecular interactions, regulatory mechanisms, and downstream target genes in vitro. Notably, INSM1 has gained clinical interest as a sensitive biomarker for diagnosing neuroendocrine tumors (e.g., neuroblastoma, medullary thyroid carcinoma) due to its specific expression pattern in these malignancies.
In cancer research, recombinant INSM1 helps investigate tumor progression mechanisms, including its dual role as both a tumor suppressor and oncogene depending on cellular context. Additionally, it serves as a tool for exploring regenerative medicine strategies, particularly in differentiating stem cells into neuroendocrine lineages. Its conserved functional domains across species make it valuable for cross-species developmental studies. Current challenges include fully elucidating its contradictory roles in cancer and optimizing its therapeutic potential while minimizing off-target effects in gene regulation studies.
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