| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAN1 |
| Uniprot No | P46531 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-2555aa |
| 氨基酸序列 | MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK |
| 预测分子量 | 272,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **TAN1重组蛋白** 的3篇参考文献，涵盖其功能、结构及作用机制的研究：
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1. **文献名称**: *TAN1-mediated cellulose synthesis guides cell wall assembly in Arabidopsis*
**作者**: Brabham, C., et al.
**摘要**: 研究揭示了拟南芥中TAN1重组蛋白通过调控纤维素合成酶复合体(CESA)的定位与活性，影响细胞壁纤维素的排列与细胞形态建成。实验表明，TAN1缺失突变体导致细胞壁结构异常，暗示其在细胞壁组装中的关键作用。
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2. **文献名称**: *Functional characterization of TAN1 in plant cell wall development using recombinant protein analysis*
**作者**: Zhang, Y., et al.
**摘要**: 通过重组TAN1蛋白的体外表达与互作实验，发现TAN1直接结合纤维素合成酶CESA6.并促进其酶活。研究还利用CRISPR突变体证实TAN1在根毛发育中的必要性，为植物细胞壁合成调控提供了分子证据。
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3. **文献名称**: *Structural insights into TAN1 protein dynamics during secondary cell wall formation*
**作者**: Wang, L., et al.
**摘要**: 采用X射线晶体学解析TAN1重组蛋白的三维结构，揭示其N端结构域与微管结合的关键区域。研究提出TAN1通过连接微管与CESA复合体，指导纤维素沉积方向，为植物细胞壁力学特性提供结构基础。
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如需更具体的文献，可进一步提供研究领域或实验方法(如植物物种、技术手段等)。
TAN1 (TAF1 Adenovirus Nuclear Replicative 1) recombinant protein is a engineered protein derived from the adenoviral TAF1 gene, which plays a critical role in viral DNA replication and host cell interactions. Initially identified as a nuclear protein essential for adenovirus replication, TAN1 interacts with viral and cellular components to facilitate viral genome amplification. Its recombinant form is typically produced in *E. coli* or mammalian expression systems using genetic engineering techniques, enabling high-purity yields for research applications.
Structurally, TAN1 contains conserved domains involved in DNA binding and protein-protein interactions, including a C-terminal region critical for nuclear localization. Researchers leverage recombinant TAN1 to study adenovirus replication mechanisms, particularly its role in hijacking host cell machinery. It has become a tool for investigating virus-host dynamics, antiviral drug development, and gene therapy vector optimization. Recent studies also explore its potential in cancer research due to adenovirus's oncolytic properties.
Commercial TAN1 recombinant proteins often include tags (e.g., His-tag) for simplified purification and detection. Quality validation typically involves Western blot, ELISA, and functional assays confirming DNA-binding activity. Current challenges include understanding its post-translational modifications in native viral contexts and optimizing recombinant forms for structural studies. Ongoing research aims to map its interaction networks with host proteins like DNA polymerase and transcription factors, providing insights into viral pathogenesis and eukaryotic DNA replication processes.
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