Recombinant Human RVT protein

中文名： 白藜芦醇(RVT)牛血清白蛋白偶联物
别名： RVT；Endogenous retrovirus group K member 19 Pol protein

货号: PA2000-1009

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	RVT
Uniprot No	Q9WJR5
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-959aa
氨基酸序列	NKSKKRRNRVSFLGAATVEPPKPIPLTWKTEKPVWVNQWPLPKQKLEALHLLANEQLEKGHIEPSFSPWNSPVFVIQKKSGKWRMLTDLRAVNAVNAVIQPMGPLQPGLPSLAMIPKDWPLIIIDLKDCFFTIPLAEQDCEKFAFTIPAINNKEPATRFQWKVLPQGMLNSPTICQTFVGRALQPVREKFSDCYIIHYIDDILCAAEMKDKLIDCYTFLQAEVANAGLAIASDKIQTSTPFHYLEMQIENRKIKPPKIEIRKDTLKTLNDFQKLLGDINWIRPTLGIPTYAMSNLFSILRGDSDLNSKRMLTPEATKEIKLVEEKIQSAQINRIDPLAPLQLLIFATAHSPTGIIIQNTDLVEWSFLPHSTVKTFTLYLDQMATLIGQTRLRIIKLCGNDPDKIVVPLTKEQVRQAFINSGAWQIGLANFVGIIDNHYPKTKIFQFLKMTTWILPKITRREPLENALTVFTDGSSNGKAAYTGPKERVIKTQYQSAQRAELVAVITVLQDFDQPINIISDSAYVVQATRDVETALIKYSMDDQLNQLFNLLQQTVRKRNFPFYITHIRAHTNLPGPLTKANEQADLLVSSALIKAQELHALTHVNVAGLKNKFDVTWKQAKDIVQHCTQCQVLHLPTQEAGVNPRGLCPNALWQMDVTHVSSFGRLSYIHVTVDTYSHFIWATCQTGESTSHVKKHLLSCFAVMGVPEKIKTDNGPGYCSKAFQKFLSQWKISHTTGIPYNSQGQAIVERTNRTLKTQLVKQKEGGDSKECTTPQMQLNLALYTLNFLNIYRNQTTTSAEQHLTGKKNSPHEGKLIWWKDNKNKTWEIGKVITWGRGFACVSPGENQLPVWIPTRHLKFYNEPIGDAKKSTSAETETPQSSTVDSQDEQNGDVRRTDEVAIHQESRAADLGTTKEADAVSYKISREHKGDTNPREYAACGLDDCINGGKSPYACRSSCS
预测分子量	108,1 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3-4条关于重组逆转录酶(RVT)的示例参考文献(基于学术文献的典型结构，供参考)：

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1. **文献名称**：*High-yield expression and purification of recombinant HIV-1 reverse transcriptase in E. coli*

**作者**：Johnson A, Smith B, et al.

**摘要**：本研究报道了在大肠杆菌中高效表达HIV-1逆转录酶的重组方法，通过密码子优化和温度诱导策略提高蛋白产量，并利用亲和层析纯化获得高纯度、高酶活性的重组蛋白，为病毒研究提供可靠工具。

2. **文献名称**：*Thermostable mutant reverse transcriptase with enhanced processivity for cDNA synthesis*

**作者**：Chen L, Wang Y, et al.

**摘要**：通过定点突变改造重组逆转录酶，获得热稳定性与持续合成能力显著提升的突变体。实验表明该突变体在高温(50°C)下仍保持活性，适用于长链RNA模板的cDNA合成。

3. **文献名称**：*Structural and functional analysis of a novel recombinant reverse transcriptase from retrovirus-like elements*

**作者**：Martinez R, et al.

**摘要**：从类逆转录病毒元件中克隆新型逆转录酶基因，通过X射线晶体学解析其结构，揭示其独特的底物结合域，并验证其在体外逆转录活性，拓展了对逆转录酶多样性的理解。

4. **文献名称**：*Application of recombinant reverse transcriptase in single-cell RNA sequencing*

**作者**：Kim S, et al.

**摘要**：评估重组逆转录酶在单细胞RNA测序中的性能，比较不同商业酶在低模板量下的效率与准确性，提出优化方案以提高转录组覆盖度。

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**说明**：以上文献为示例性质，实际引用时需检索具体数据库(如PubMed、Web of Science)获取真实发表的论文。若需真实文献，可尝试关键词："recombinant reverse transcriptase" + "expression"/"application"/"engineering"等。

背景信息

Recombinant proteins, including RVT (recombinant viral transfer) proteins, represent a cornerstone of modern biotechnology, enabling the production of tailored proteins for research, therapeutic, and industrial applications. The concept stems from recombinant DNA technology developed in the 1970s, which allows the insertion of target genes into host organisms (e.g., bacteria, yeast, or mammalian cells) to express specific proteins. RVT proteins, often derived from viral components, are engineered to retain functional domains while eliminating pathogenic properties, making them safe and versatile tools.

A key application of RVT proteins lies in virology and vaccine development. For instance, recombinant viral envelope or capsid proteins are used to study viral entry mechanisms or as antigenic components in subunit vaccines. The COVID-19 pandemic highlighted their importance, with recombinant spike proteins serving as critical antigens in several vaccines. Beyond immunology, RVT proteins are employed in gene therapy vectors, where engineered viral proteins facilitate targeted gene delivery without replication risks.

Production typically involves cloning viral gene segments into expression vectors, followed by purification via chromatography. Challenges include ensuring proper protein folding, post-translational modifications, and scalability. Advances in cell-free systems and AI-driven protein design now enhance yield and functionality.

Ethical and safety considerations remain paramount, particularly regarding unintended immune responses or genetic instability. Despite these hurdles, RVT recombinant proteins continue to revolutionize medicine, offering precise solutions for diagnostics, therapeutics, and synthetic biology. Their adaptability positions them at the forefront of addressing emerging infectious diseases and genetic disorders.