| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCOA6 |
| Uniprot No | Q14686 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-2063aa |
| 氨基酸序列 | MVLDDLPNLEDIYTSLCSSTMEDSEMDFDSGLEDDDTKSDSILEDSTIFVAFKGNIDDKDFKWKLDAILKNVPNLLHMESSKLKVQKVEPWNSVRVTFNIPREAAERLRILAQSNNQQLRDLGILSVQIEGEGAINLALAQNRSQDVRMNGPMGAGNSVRMEAGFPMASGPGIIRMNNPATVMIPPGGNVSSSMMAPGPNPELQPRTPRPASQSDAMDPLLSGLHIQQQSHPSGSLAPPHHPMQPVSVNRQMNPANFPQLQQQQQQQQQQQQQQQQQQQQQQQQQLQARPPQQHQQQQPQGIRPQFTAPTQVPVPPGWNQLPSGALQPPPAQGSLGTMTANQGWKKAPLPGPMQQQLQARPSLATVQTPSHPPPPYPFGSQQASQAHTNFPQMSNPGQFTAPQMKSLQGGPSRVPTPLQQPHLTNKSPASSPSSFQQGSPASSPTVNQTQQQMGPRPPQNNPLPQGFQQPVSSPGRNPMVQQGNVPPNFMVMQQQPPNQGPQSLHPGLGGMPKRLPPGFSAGQANPNFMQGQVPSTTATTPGNSGAPQLQANQNVQHAGGQGAGPPQNQMQVSHGPPNMMQPSLMGIHGNMNNQQAGTSGVPQVNLSNMQGQPQQGPPSQLMGMHQQIVPSQGQMVQQQGTLNPQNPMILSRAQLMPQGQMMVNPPSQNLGPSPQRMTPPKQMLSQQGPQMMAPHNQMMGPQGQVLLQQNPMIEQIMTNQMQGNKQQFNTQNQSNVMPGPAQIMRGPTPNMQGNMVQFTGQMSGQMLPQQGPVNNSPSQVMGIQGQVLRPPGPSPHMAQQHGDPATTANNDVSLSQMMPDVSIQQTNMVPPHVQAMQGNSASGNHFSGHGMSFNAPFSGAPNGNQMSCGQNPGFPVNKDVTLTSPLLVNLLQSDISAGHFGVNNKQNNTNANKPKKKKPPRKKKNSQQDLNTPDTRPAGLEEADQPPLPGEQGINLDNSGPKLPEFSNRPPGYPSQPVEQRPLQQMPPQLMQHVAPPPQPPQQQPQPQLPQQQQPPPPSQPQSQQQQQQQQQMMMMLMMQQDPKSVRLPVSQNVHPPRGPLNPDSQRMPMQQSGSVPVMVSLQGPASVPPSPDKQRMPMPVNTPLGSNSRKMVYQESPQNPSSSPLAEMASLPEASGSEAPSVPGGPNNMPSHVVLPQNQLMMTGPKPGPSPLSATQGATPQQPPVNSLPSSHGHHFPNVAAPTQTSRPKTPNRASPRPYYPQTPNNRPPSTEPSEISLSPERLNASIAGLFPPQINIPLPPRPNLNRGFDQQGLNPTTLKAIGQAPSNLTMNPSNFATPQTHKLDSVVVNSGKQSNSGATKRASPSNSRRSSPGSSRKTTPSPGRQNSKAPKLTLASQTNAALLQNVELPRNVLVSPTPLANPPVPGSFPNNSGLNPQNSTVSVAAVGGVVEDNKESLNVPQDSDCQNSQSRKEQVNIELKAVPAQEVKMVVPEDQSKKDGQPSDPNKLPSVEENKNLVSPAMREAPTSLSQLLDNSGAPNVTIKPPGLTDLEVTPPVVSGEDLKKASVIPTLQDLSSSKEPSNSLNLPHSNELCSSLVHPELSEVSSNVAPSIPPVMSRPVSSSSISTPLPPNQITVFVTSNPITTSANTSAALPTHLQSALMSTVVTMPNAGSKVMVSEGQSAAQSNARPQFITPVFINSSSIIQVMKGSQPSTIPAAPLTTNSGLMPPSVAVVGPLHIPQNIKFSSAPVPPNALSSSPAPNIQTGRPLVLSSRATPVQLPSPPCTSSPVVPSHPPVQQVKELNPDEASPQVNTSADQNTLPSSQSTTMVSPLLTNSPGSSGNRRSPVSSSKGKGKVDKIGQILLTKACKKVTGSLEKGEEQYGADGETEGQGLDTTAPGLMGTEQLSTELDSKTPTPPAPTLLKMTSSPVGPGTASAGPSLPGGALPTSVRSIVTTLVPSELISAVPTTKSNHGGIASESLAGGLVEEKVGSHPELLPSIAPSQNLVSKETSTTALQASVARPELEVNAAIVSGQSSEPKEIVEKSKIPGRRNSRTEEPTVASESVENGHRKRSSRPASASSSTKDITSAVQSKRRKSK |
| 预测分子量 | 219,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCOA6重组蛋白的3篇参考文献示例(文献信息为模拟虚构，仅供参考)：
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1. **文献名称**：*Structural characterization of recombinant NCOA6 and its role in nuclear receptor coactivation*
**作者**：Zhang Y, et al.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组NCOA6蛋白，并利用X射线晶体学解析其C端结构域的三维结构。实验表明，重组NCOA6能够增强甲状腺激素受体(TR)的转录活性，揭示了其通过特定结构域介导共激活作用的分子机制。
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2. **文献名称**：*NCOA6 recombinant protein facilitates PPARγ-dependent adipogenesis in vitro*
**作者**：Lee S, et al.
**摘要**：通过昆虫细胞表达系统获得高纯度NCOA6重组蛋白，并证明其在3T3-L1前脂肪细胞分化中与PPARγ协同调控脂质代谢相关基因。研究为代谢综合征中NCOA6的功能提供了体外实验证据。
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3. **文献名称**：*Development of a NCOA6 recombinant protein-based assay for screening coactivator inhibitors*
**作者**：Wang H, et al.
**摘要**：利用哺乳动物细胞表达的重组NCOA6蛋白，建立了一种高通量药物筛选平台，鉴定出两种小分子化合物可特异性阻断NCOA6与雌激素受体(ERα)的结合，为靶向核受体通路的抗癌药物研发提供了新策略。
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注：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。
Nuclear receptor coactivator 6 (NCOA6), also known as ASC-2 or RAP80. is a multifunctional transcriptional coactivator that plays a critical role in regulating gene expression through interactions with nuclear receptors and other transcription factors. It belongs to the p160 coactivator family and functions as a scaffold protein, mediating the assembly of large transcriptional complexes involving histone-modifying enzymes (e.g., CBP/p300. CARM1) and chromatin-remodeling factors. NCOA6 enhances the transcriptional activity of nuclear receptors such as estrogen receptor (ER), retinoic acid receptor (RAR), and peroxisome proliferator-activated receptors (PPARs), thereby influencing diverse biological processes, including hormone signaling, metabolism, cell differentiation, and embryonic development.
Structurally, NCOA6 contains multiple LXXLL motifs (nuclear receptor-binding domains), an intrinsic transcriptional activation domain, and regions that recruit co-regulatory proteins. Its involvement in key signaling pathways, such as Wnt/β-catenin and NF-κB, underscores its regulatory versatility. Dysregulation of NCOA6 has been implicated in cancer progression, particularly in hormone-dependent cancers (e.g., breast, prostate) and hepatocellular carcinoma, where it may promote tumor growth, metastasis, or therapy resistance through aberrant gene activation.
Recombinant NCOA6 protein is engineered using expression systems like E. coli or mammalian cells, often tagged with affinity markers (e.g., His, GST) for purification and detection. This tool enables in vitro studies to dissect molecular interactions, enzymatic activities in chromatin modification, and screening of therapeutic compounds targeting NCOA6-associated pathways. Its role as a coactivator in nuclear receptor signaling also positions it as a potential biomarker or drug target for metabolic disorders, cancers, and developmental abnormalities. Ongoing research aims to elucidate its context-dependent functions and therapeutic modulation.
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