Recombinant Human FABP8 protein

中文名： 髓鞘型脂肪酸结合蛋白(FABP8)重组蛋白
别名： FABP8；Myelin P2 protein

货号: PA2000-36DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	FABP8
Uniprot No	P02689
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-132aa
氨基酸序列	MSNKFLGTWKLVSSENFDDYMKALGVGLATRKLGNLAKPTVIISKKGDIITIRTESTFKNTEISFKLGQEFEETTADNRKTKSIVTLQRGSLNQVQRWDGKETTIKRKLVNGKMVAECKMKGVVCTRIYEKV
预测分子量	41.9kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于FABP8重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：

---

1. **文献名称**：*"Expression and purification of recombinant human FABP8 in a prokaryotic system"*

**作者**：Zhang Y, et al.

**摘要**：本研究成功构建了大肠杆菌表达系统生产重组人FABP8蛋白，通过优化诱导条件和亲和层析纯化获得高纯度蛋白，并证实其与天然蛋白的脂肪酸结合活性一致。

2. **文献名称**：*"Crystal structure of recombinant FABP8 complexed with docosahexaenoic acid (DHA)"*

**作者**：Tanaka K, et al.

**摘要**：解析了重组FABP8与DHA结合的晶体结构，揭示了其结合口袋的关键氨基酸残基，为研究FABP8在神经细胞脂质运输中的作用机制提供结构基础。

3. **文献名称**：*"Recombinant FABP8 modulates inflammatory responses in peripheral nerve injury models"*

**作者**：Müller S, et al.

**摘要**：通过体外实验证明，重组FABP8蛋白能够抑制施万细胞炎症因子释放，并减轻坐骨神经损伤模型中的炎症反应，提示其潜在治疗神经病变的应用价值。

---

如需实际文献，建议通过**PubMed**或**Web of Science**搜索关键词“FABP8 recombinant protein”或“PMP2 recombinant expression”获取准确信息。

背景信息

Fatty acid-binding protein 8 (FABP8), also known as peripheral myelin protein 2 (PMP2) or myelin fatty acid-binding protein (MFABP), is a small cytoplasmic protein belonging to the fatty acid-binding protein (FABP) family. These proteins play critical roles in lipid metabolism, cellular signaling, and fatty acid transport. FABP8 is primarily expressed in peripheral nervous system tissues, particularly in Schwann cells, where it contributes to the formation and maintenance of myelin sheaths around peripheral axons. Its ability to bind long-chain fatty acids (LCFAs) facilitates the intracellular transport of hydrophobic ligands, supporting membrane biosynthesis and energy metabolism in myelinating cells.

Structurally, FABP8 contains a conserved β-barrel motif with a helical cap region, forming a hydrophobic pocket for ligand binding. This 14-15 kDa protein is encoded by the *PMP2* gene in humans and shares sequence homology with other FABP isoforms. Studies suggest FABP8 regulates lipid homeostasis by coordinating fatty acid uptake, trafficking, and utilization in Schwann cells, which is vital for axonal integrity and nerve conduction. Dysregulation of FABP8 has been linked to peripheral neuropathies, including Charcot-Marie-Tooth disease type 1B (CMT1B), where mutations in *PMP2* disrupt myelin stability.

Recombinant FABP8 is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Purified recombinant proteins enable investigations into lipid-protein interactions, structural dynamics, and disease mechanisms. Its applications extend to neurological research, drug discovery for demyelinating disorders, and biomarker development. Recent work also explores its role in neuroinflammation and potential cross-talk with other lipid-binding proteins in neurodegenerative conditions. As a model protein, recombinant FABP8 provides insights into lipid-mediated cellular processes and therapeutic targeting in peripheral nerve diseases.