Recombinant Human UCN3 protein

中文名： 尿皮质素3(UCN3)重组蛋白
别名： UCN3；SPC；Urocortin-3

货号: PA1000-9453

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	UCN3
Uniprot No	Q969E3
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	119-161aa
氨基酸序列	KFTLSLDVPTNIMNLLFNIAKAKNLRAQAAANAHLMAQIGRKK
预测分子量	20.1 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于UCN3重组蛋白的3篇参考文献(基于公开研究整理，非真实文献，仅供参考)：

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1. **文献名称**：*Expression and Purification of Recombinant Urocortin 3 in E. coli for Functional Studies*

**作者**：Chen L, et al.

**摘要**：该研究报道了在大肠杆菌中高效表达重组UCN3蛋白的优化策略，采用His标签纯化系统，并通过质谱和圆二色光谱验证其正确折叠。重组UCN3在体外实验中显示出与CRFR2受体的特异性结合活性。

2. **文献名称**：*Structural Characterization of Recombinant UCN3 and Its Role in Glucose Homeostasis*

**作者**：Smith K, et al.

**摘要**：通过重组技术获得人源UCN3蛋白，结合核磁共振(NMR)解析其溶液结构，并发现其在糖尿病小鼠模型中能显著改善胰岛素敏感性，提示其潜在的治疗代谢疾病的应用价值。

3. **文献名称**：*Recombinant UCN3 Attenuates Anxiety-like Behavior via CRFR2 Signaling in Rodent Models*

**作者**：Wang Y, et al.

**摘要**：研究利用重组UCN3蛋白探究其对焦虑行为的影响，结果表明其通过激活CRFR2受体通路降低小鼠应激反应，为开发新型抗焦虑药物提供了实验依据。

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如需具体文献，建议在PubMed或Web of Science中以“recombinant urocortin 3”或“UCN3 expression”为关键词检索最新研究。

背景信息

Urocortin 3 (UCN3), a member of the corticotropin-releasing factor (CRF) neuropeptide family, plays a critical role in modulating stress responses, energy homeostasis, and emotional regulation. Identified in the early 2000s, UCN3 selectively binds to the type 2 CRF receptor (CRFR2), distinguishing it from other family members like CRF and urocortin 1. which primarily activate CRFR1. This specificity links UCN3 to unique physiological pathways, including glucose metabolism, appetite suppression, and anxiety-related behaviors. It is expressed in discrete brain regions (e.g., hypothalamic nuclei, amygdala) and peripheral tissues (pancreas, gastrointestinal tract), suggesting both central and peripheral functions.

Recombinant UCN3 protein is engineered using genetic cloning techniques, often expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper post-translational modifications. Purification methods typically involve affinity chromatography, yielding high-purity bioactive peptides. As a research tool, recombinant UCN3 facilitates studies on CRFR2 signaling mechanisms, aiding the exploration of metabolic disorders (e.g., diabetes, obesity) and neuropsychiatric conditions (e.g., anxiety, depression). Its potential therapeutic applications include targeting CRFR2 to modulate stress adaptation or appetite circuits, though clinical translation remains exploratory. Challenges include optimizing stability and delivery methods, as well as elucidating its complex interplay with other CRF family peptides in disease contexts. Current research continues to unravel its dual role in stress resilience and metabolic regulation, positioning UCN3 as a promising yet understudied target in translational medicine.