Recombinant Human PGLYRP2 protein

中文名： 肽聚糖识别蛋白2(PGLYRP2)重组蛋白
别名： PGLYRP2；PGLYRPL；PGRPL；N-acetylmuramoyl-L-alanine amidase

货号: PA1000-9205

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PGLYRP2
Uniprot No	Q96PD5
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	22-576aa
氨基酸序列	SLPLLMDSVIQALAELEQKVPAAKTRHTASAWLMSAPNSGPHNRLYHFLLGAWSLNATELDPCPLSPELLGLTKEVARHDVREGKEYGVVLAPDGSTVAVEPLLAGLEAGLQGRRVINLPLDSMAAPWETGDTFPDVVAIAPDVRATSSPGLRDGSPDVTTADIGANTPDATKGCPDVQASLPDAKAKSPPTMVDSLLAVTLAGNLGLTFLRGSQTQSHPDLGTEGCWDQLSAPRTFTLLDPKASLLTMAFLNGALDGVILGDYLSRTPEPRPSLSHLLSQYYGAGVARDPGFRSNFRRQNGAALTSASILAQQVWGTLVLLQRLEPVHLQLQCMSQEQLAQVAANATKEFTEAFLGCPAIHPRCRWGAAPYRGRPKLLQLPLGFLYVHHTYVPAPPCTDFTRCAANMRSMQRYHQDTQGWGDIGYSFVVGSDGYVYEGRGWHWVGAHTLGHNSRGFGVAIVGNYTAALPTEAALRTVRDTLPSCAVRAGLLRPDYALLGHRQLVRTDCPGDALFDLLRTWPHFTATVKPRPARSVSKRSRREPPPRTLPATDLQ
预测分子量	76.0kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

1. **"Peptidoglycan Recognition Proteins Kill Bacteria by Inducing Oxidative, Thiol, and Metal Stress"**

*Authors: Kashyap DR, Wang M, Liu LH, Boons GJ, Gupta D, Dziarski R*

**摘要**：该研究探讨了PGLYRP2重组蛋白的抗菌机制，发现其通过诱导细菌氧化应激、巯基应激及金属离子失衡导致细菌死亡，揭示了其在先天免疫中的直接杀菌作用。

2. **"Recombinant PGLYRP2 Protein Modulates Inflammatory Responses in Murine Macrophages"**

*Authors: Roy S, Gupta D, Dziarski R*

**摘要**：研究利用重组PGLYRP2蛋白处理小鼠巨噬细胞，发现其通过抑制TLR/NF-κB通路降低促炎因子(如TNF-α、IL-6)表达，提示其在调控过度炎症中的潜在治疗价值。

3. **"Expression and Characterization of Human PGLYRP2 in a Mammalian Cell System"**

*Authors: Zhang Y, Li X, Wang H*

**摘要**：成功在HEK293细胞中表达功能性重组人源PGLYRP2蛋白，优化纯化工艺并验证其对肽聚糖的水解活性，为后续疾病模型研究提供高纯度蛋白来源。

4. **"PGLYRP2 Deficiency Exacerbates Colitis via Gut Microbiota Dysbiosis"**

*Authors: Chen L, Liu Z, Hooper LV*

**摘要**：通过构建PGLYRP2基因敲除小鼠，发现其缺失导致肠道菌群紊乱并加重结肠炎，而外源重组PGLYRP2蛋白可恢复菌群平衡并缓解炎症，表明其在肠道免疫稳态中的作用。

背景信息

**Background of PGLYRP2 Recombinant Protein**

Peptidoglycan recognition protein 2 (PGLYRP2) is a member of the PGLYRP family, a group of pattern recognition receptors (PRRs) that specifically bind to peptidoglycan (PGN), a key component of bacterial cell walls. Unlike other PGLYRPs (PGLYRP1. 3. 4), which exhibit direct bactericidal activity, PGLYRP2 functions as an N-acetylmuramoyl-L-alanine amidase, enzymatically cleaving PGN to modulate immune responses. This intracellular protein is constitutively expressed in the liver and secreted into the bloodstream, playing a regulatory role in innate immunity by preventing excessive inflammation triggered by bacterial PGN.

Recombinant PGLYRP2 is produced using biotechnological systems (e.g., *E. coli* or mammalian cell expression) to generate purified, functional protein for research and therapeutic applications. Its enzymatic activity helps degrade pro-inflammatory PGN fragments, making it a critical subject in studies of immune homeostasis, inflammatory diseases, and host-microbe interactions. Dysregulation of PGLYRP2 has been linked to chronic inflammatory conditions, such as inflammatory bowel disease (IBD) and rheumatoid arthritis, highlighting its potential as a therapeutic target.

Research on recombinant PGLYRP2 also explores its diagnostic utility, as altered levels correlate with bacterial infections or dysbiosis. Additionally, it serves as a tool to investigate PGN metabolism and its impact on gut microbiota composition. Recent studies suggest its involvement in modulating autophagy and apoptosis, further expanding its biological significance beyond innate immunity. Overall, PGLYRP2 recombinant protein is pivotal in advancing understanding of immune regulation and developing strategies to manage inflammation-related disorders.