| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYN1 |
| Uniprot No | P17600 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 113-420aa |
| 氨基酸序列 | SRVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPR |
| 预测分子量 | 41.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYN1重组蛋白的3篇参考文献摘要简述：
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1. **文献名称**：*"Synapsin I regulation of neurotransmitter release: A role for its interactions with the ATP-binding cassette transporter of synaptic vesicles?"*
**作者**：Hilfiker S, et al.
**摘要**：该研究通过重组Synapsin I蛋白的体外实验，揭示了其通过调控突触囊泡的ATP依赖型运输影响神经递质释放的分子机制，证实了其C端结构域在囊泡动态调控中的关键作用。
2. **文献名称**：*"The synapsins: Multitask modulators of neuronal development and synaptic plasticity"*
**作者**：Cesca F, et al.
**摘要**：这篇综述系统总结了Synapsin家族(包括SYN1)在突触可塑性与神经发育中的多重功能，特别指出重组Synapsin蛋白技术为解析其磷酸化依赖的突触囊泡锚定机制提供了重要工具。
3. **文献名称**：*"Synapsin I is structurally conserved in evolution and interacts with molecules involved in synaptic vesicle clustering"*
**作者**：Hosaka M, Südhof TC.
**摘要**：研究者通过重组表达SYN1蛋白，鉴定了其高度保守的N端结构域与突触囊泡聚集相关蛋白(如Rab3A)的特异性结合能力，为解释其在突触前功能的结构基础提供了证据。
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**备注**：上述文献为示例性质，实际文献标题与细节可能存在差异。建议通过PubMed或Web of Science以“Synapsin 1 recombinant”为关键词检索最新研究。
SYN1. or Synapsin-1. is a neuron-specific phosphoprotein critical for regulating synaptic vesicle dynamics in presynaptic terminals. It belongs to the synapsin family, which modulates neurotransmitter release by tethering synaptic vesicles to the actin cytoskeleton, thereby controlling their availability for exocytosis. SYN1 is encoded by the SYN1 gene and contains conserved domains that interact with vesicles, cytoskeletal elements, and other synaptic proteins. Its function is regulated by phosphorylation, which influences synaptic plasticity and neuronal communication. Dysregulation of SYN1 has been linked to neurological disorders, including epilepsy, autism spectrum disorders, and schizophrenia.
Recombinant SYN1 protein is produced using biotechnological platforms (e.g., E. coli or mammalian expression systems) to enable controlled studies of its molecular interactions and pathological mechanisms. Purified recombinant SYN1 retains functional domains, allowing researchers to investigate its role in vesicle clustering, neurotransmitter release, and synapse formation. It serves as a tool for in vitro assays, structural studies, and antibody development. Notably, mutations in SYN1 identified in neurodevelopmental disorders can be replicated in recombinant forms to study their biochemical and cellular effects.
Research on recombinant SYN1 has advanced understanding of synaptic dysfunction in disease models and potential therapeutic targets. For example, studies using recombinant SYN1 have elucidated how phosphorylation defects impair vesicle trafficking or how pathogenic mutations disrupt protein-protein interactions. Additionally, it supports drug screening platforms aiming to restore synaptic vesicle homeostasis. As a biomarker candidate, recombinant SYN1 aids in detecting autoantibodies in autoimmune encephalitis. Its applications extend to neuroscience, pharmacology, and diagnostics, highlighting its importance in bridging molecular insights with clinical translation.
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