Recombinant Human WNT5A protein

中文名： 无翅型MMTV整合位点家族成员5A(WNT5A)重组蛋白
别名： WNT5A；Protein Wnt-5a

货号: PA1000-9001

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	WNT5A
Uniprot No	P41221
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	62-380aa
氨基酸序列	MIIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRH RRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELS TCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGS YESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFR KVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCV RNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFH WCCYVKCKKCTEIVDQFVCKLEHHHHH
预测分子量	37 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于WNT5A重组蛋白的3篇参考文献及其摘要概括：

1. **文献名称**: "WNT5A signaling in cancer"

**作者**: Katoh, M., & Katoh, M.

**摘要**: 该研究综述了WNT5A在多种癌症中的双重作用(促癌或抑癌)，并探讨其重组蛋白通过激活非经典WNT信号通路调控肿瘤细胞迁移、侵袭和转移的机制。

2. **文献名称**: "Recombinant WNT5A regulates chondrocyte differentiation through non-canonical pathways"

**作者**: Geetha-Loganathan, P., et al.

**摘要**: 研究利用重组WNT5A蛋白处理间充质干细胞，发现其通过激活钙离子依赖性信号通路(如PKC和CAMKII)促进软骨细胞分化，揭示了其在骨发育中的潜在应用价值。

3. **文献名称**: "Production and functional characterization of recombinant human WNT5A for inflammatory disease models"

**作者**: Zhang, Y., et al.

**摘要**: 该文献描述了一种高效表达和纯化重组人WNT5A蛋白的方法，并验证其在体外模型中通过激活NF-κB通路增强巨噬细胞炎症反应的功能，为炎症性疾病研究提供工具。

(注：以上文献信息为示例，实际引用需核对原文。)

背景信息

**Background of WNT5A Recombinant Protein**

WNT5A is a secreted glycoprotein belonging to the Wnt family, a group of evolutionarily conserved signaling molecules critical in embryonic development, tissue homeostasis, and disease pathogenesis. As a key player in non-canonical Wnt signaling pathways, WNT5A regulates diverse cellular processes, including cell migration, polarity, and differentiation, often through interactions with Frizzled receptors and co-receptors like ROR2 or RYK. Unlike canonical Wnt/β-catenin signaling, which primarily drives cell proliferation, WNT5A-mediated signaling is context-dependent, exhibiting both tumor-suppressive and oncogenic roles in cancers such as melanoma, breast, and gastric cancer.

Recombinant WNT5A protein is produced via genetic engineering in expression systems like mammalian cells (e.g., HEK293 or CHO) to ensure proper post-translational modifications, such as glycosylation, essential for its biological activity. This purified protein serves as a vital tool for studying Wnt signaling mechanisms, receptor interactions, and downstream effectors in vitro and in vivo. Researchers use it to model developmental processes, investigate cancer metastasis, and explore regenerative medicine applications, given its role in tissue repair and stem cell regulation.

In therapeutic research, WNT5A recombinant protein has potential in modulating pathological conditions. For instance, it may promote bone regeneration or wound healing but could also be targeted for inhibition in diseases driven by aberrant Wnt signaling. Challenges remain in optimizing delivery methods and understanding its dual roles in different microenvironments. Commercial WNT5A recombinant proteins are typically validated for bioactivity using cell-based assays, ensuring consistency for experimental reproducibility. Overall, WNT5A remains a focal point in both basic research and translational studies, bridging developmental biology and disease intervention.