Recombinant Human WNT4 protein

中文名： 无翅型MMTV整合位点家族成员4(WNT4)重组蛋白
别名： WNT4；Protein Wnt-4

货号: PA1000-9000

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	WNT4
Uniprot No	P56705-1
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	23-351aa
氨基酸序列	SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQ LAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAF AVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDV RERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWR AVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVY LEPSPDFCEQDMRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVEL AERCSCKFHWCCFVKCRQCQRLVELHTCR
预测分子量	53 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇与WNT4重组蛋白相关的文献概览(注：文献为示例性内容，实际引用请核实原文)：

1. **《WNT4重组蛋白在卵巢发育中的功能研究》**

*作者：Vainio S et al.*

摘要：研究通过在大肠杆菌中表达重组WNT4蛋白，证实其能激活β-catenin信号通路，并诱导小鼠胚胎性腺分化为卵巢组织，揭示了WNT4在哺乳动物性别决定中的关键作用。

2. **《重组WNT4蛋白促进间充质干细胞成骨分化》**

*作者：Zhang Y et al.*

摘要：利用哺乳动物细胞系统表达人源WNT4重组蛋白，发现其通过调控RUNX2基因增强间充质干细胞的成骨分化能力，为骨再生治疗提供了潜在策略。

3. **《WNT4重组蛋白抑制乳腺癌细胞迁移的机制》**

*作者：Bissonnette N et al.*

摘要：研究发现重组WNT4蛋白能通过拮抗Wnt/β-catenin通路抑制乳腺癌细胞侵袭，并降低MMP-9表达，提示其作为转移性乳腺癌治疗的分子靶点。

4. **《昆虫细胞表达系统制备高活性WNT4重组蛋白》**

*作者：Willert K et al.*

摘要：优化了昆虫杆状病毒系统表达WNT4的条件，获得高纯度、高生物活性的重组蛋白，并通过体外实验验证其对上皮细胞极化的调控功能。

(注：以上文献信息为模拟生成，实际研究请参考PubMed/NCBI等数据库的权威论文。)

背景信息

**Background of WNT4 Recombinant Protein**

WNT4. a member of the Wnt family of secreted glycoproteins, plays pivotal roles in embryonic development, tissue homeostasis, and cell signaling. It specifically activates the β-catenin-dependent (canonical) and -independent (non-canonical) pathways, regulating processes such as cell proliferation, differentiation, and polarity. WNT4 is critically involved in organogenesis, including kidney development, female reproductive tract formation, and bone morphogenesis. Notably, it is essential for Müllerian duct differentiation in mammals and has been linked to disorders of sex development (DSD) when mutated.

Recombinant WNT4 protein is engineered to mimic the native protein's biological activity, typically produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications, such as glycosylation and lipidylation, which are crucial for its stability and receptor binding. The recombinant form enables controlled in vitro and in vivo studies, bypassing challenges associated with endogenous protein isolation.

Research applications include investigating WNT4's role in sex determination, tissue regeneration (e.g., kidney repair), and cancer biology, where dysregulated Wnt signaling is common. It also serves as a tool for exploring therapeutic strategies for conditions like endometriosis, osteoporosis, and congenital anomalies. However, challenges persist in maintaining its solubility and activity due to its hydrophobic nature, often requiring carrier proteins or specialized delivery systems.

Overall, WNT4 recombinant protein remains a vital reagent for dissecting Wnt signaling mechanisms and developing targeted interventions for developmental and degenerative diseases.